新型复合式老年痴呆小鼠模型的建立

目的建立一种新型的集衰老、铝中毒、铁中毒以及胆碱能系统损害等多因素复合式老年性痴呆（AD）动物模型,用于AD及其治疗药物的研究。方法取健康雄性昆明小鼠128只,随机分为8组,A组为空白对照组,B～H组为药物造模组。除空白对照组外,其余各组小鼠均每日腹腔注射D-半乳糖（120 mg.kg-1）,连续4周复合式造模给药。用Morris水迷宫法检查学习、训练结果;行为学实验结束后小鼠断颈椎处死,取脑组织,测小鼠脑组织中胆碱酯酶（CHE）和超氧化物歧化酶（SOD）的含量。结果模型F组（AlCl3＋SCOP）小鼠脑组织的CHE、SOD酶与空白对照组相比均有显著性差异（P〈0.01）,小鼠的逃避潜伏期和错误次数明显延长或增大。结论用多因素刺激可建立较为理想的复合式老年痴呆动物模型。

New mouse model of aged dementia induced by multi-factors

Objective To establish a new complex mouse model of aged dementia induced by D-galactose,aluminium trichloride,ferrous sulfate, and SCOP for Alzheimer Disease and its treatment.Methods A total of 128 experimental mice were divided into 8 groups randomly:group A as blank, group B to H as models with different drugs. Except group blank, other groups were given D-gal(120 mg·kg-1) for 4 weeks to form Alzheimer Disease model. The ability of remembering of the mice was studied by Morris water maze method. After sacrificing the mice,we took the brain and measured CHE and SOD activitity through colorimetry.Results The activitity of CHE and SOD in the brain in model group F(AlCl3+SCOP)was lower than that in the normal control group(P<0.01),and the escape lantency and error time were also longer than those of the blank group with the largest live number.Conclusion Multi-factors may be used to establish animal models of aged dementia.