胶质细胞源性神经营养因子促进培养的背根神经节神经元中P物质的释放

目的探测胶质细胞源性神经营养因子（GDNF）对培养的胎鼠背根神经节（DRG）神经元中P物质（SP）释放的影响作用。方法Wistar胎鼠DRG神经元分散培养72h，观察有GDNF（5ng/mL, 50ng/mL）和没有GDNF孵育的神经元活细胞生长状况,计数用微管相关蛋白2（MAP2）和4′, 6二脒基-2-苯吲哚（DAPI）双染的DRG神经元数目，用放射免疫分析法（RIA）检测辣椒素孵育前后SP的释放。结果用GDNF孵育的神经元生长状况良好，单位视野内神经元数目多于无GNDF孵育的标本，SP的基础释放量和辣椒素刺激后的释放量均高于无GDNF孵育的标本。结论GDNF可促进培养的胎鼠DRG神经元的存活，能增加培养中神经递质SP的基础释放量，可能增加辣椒素诱发神经递质SP释放的敏感性。

Glial cell line-derived neurotrophic growth factor promotes substance P release from cultured dorsal root ganglion neurons

To explore the effects of glial cell line derived neurotrophic growth factor (GDNF) on substance P (SP) released from cultured dorsal root ganglion (DRG) neurons. MethodsDRG neurons of Wistar rat embryos were cultured without or with GDNF (5ng/mL, 50ng/mL) for 72h. The living cells were observed by an inverted contrast microscope. The neurons stained by microtubule associated protein 2 (MAP2) and 4′,6-Diamidino-2-phenylindole (DAPI) fluorescent were counted. The amount of SP basal release and of SP evoked by capsaicin was determined by radio immunoassay (RIA). ResultsThe living status of DRG cells with GDNF incubation was better than that without GDNF incubation. The number of DRG neurons per visual field increased in GDNF treated cultures compared with that in cultures without GDNF. Both the amount of SP basal release and the amount of SP evoked by capsaicin significantly increased in GDNF treated cultures compared with those in cultures without GDNF. ConclusionGDNF may improve survival of cultured DRG neurons and promote SP basal release and sensitize the capsaicin evoked SP release from DRG neurons.