脂联素基因单核苷酸多态rs266729、rs2241766、rs822396与老年缺血性脑血管病亚型相关性研究

目的 探讨中国北方青岛地区汉族人群脂联素（adiponectin，ADIPOQ）基因多态性与老年缺血性脑血
管病发病的相关性。
方法 入组年龄大于60岁的老年缺血性脑血管病患者，按急性卒中治疗低分子肝素试验（Trial
of Org 10172 in Acute Stroke Treatment，TOAST）分型，选取大动脉粥样硬化性（large artery
atherosclerosis，LAA）和小动脉闭塞性（small artery occlusion，SAO）两种亚型患者，其中LAA 144例，
SAO 221例；402例同期查体者进行病例对照研究。对比两组的ADIPOQ基因多态性。
结果 rs266729（-11377C/G）的基因型分布在LAA组、SAO组与对照组3组中有显著差异（P =0.036）。
3组中两两比较显示，SAO组中rs266729（-11377C/G）GG基因型分布显著高于对照组（P =0.009）；在
隐性［P =0.004，比值比（odds ratio，OR）=2.478，95%可信区间（confidence interval，CI）=1.31～4.70］、
累加模式（P =0.003，OR 2.680，95%CI 1.39～5.15）下，rs266729（-11377C/G）基因型分布在SAO组与
对照组中也有显著差异，G 等位基因能增加SAO型缺血性脑血管病的风险，但在显性模式下差异无显
著性。rs822396（-3964A/G）、rs2241766（-45T/G）的基因型分布在LAA组、SAO组与对照组3组中均
无显著差异。
结论 rs266729（-11377C/G）G 等位基因与老年缺血性脑血管病发病相关，其G 等位基因变异可增
加老年患者SAO型缺血性脑血管病的风险。

Associations between Single-nucleotide Polymorphisms rs266729, rs2241766, rs822396 of Adiponectin Gene and TOAST Subtypes of Ischemic Cerebrovascular Disease in the Elderly

Objective To investigate the relationship between adiponectin （ADIPOQ） gene polymorphism and elderly ischemic cerebrovascular disease in Han population of Qingdao area of northern China. Methods Three hundred and sixty five patients with ischemic cerebrovascular disease who were more than 60 years old were enrolled in our study, and there were 144 cases in large artery atherosclerosis （LAA） group and 221 cases in small artery occlusion （SAO） group according to TOAST classiifcation. Four hundred and two cases who received physical examination in the same period were selected for case-control study. Gene polymorphism was analyzed by using PCR-RFLP or direct sequencing assay. Results Genotype distribution of rs266729 in the LAA group, SAO group and control group was signiifcantly different （P=0.036）. Pairwise comparisons in three groups showed that distribution of GG genotype in rs266729 was higher in the SAO group than in the control group （P=0.009）, and the same results were found in recessive and additive modes （P=0.004, P=0.003）. However, there was no signiifcant difference in the dominant mode. There was no signiifcant difference in genotype distribution of rs822396 （-3964A/G） or rs2241766 （-45T/G） among the three groups.Conclusion G allele of rs266729 （-11377C/G） was associated with incidence of ischemic cerebrovascular disease in the elderly, and the allele G can increase the risk of SAO type of ischemic cerebrovascular disease.