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Genetic characterization of an α-specific gene responsible for sexual agglutinability in Saccharomyces cerevisiae: mapping and gene dose effect
Authors:Katsunori Suzuki  Naohiko Yanagishima
Institution:(1) Biological Institute, Faculty of Science, Nagoya University, 464 Chikusa-ku, Nagoya, Japan
Abstract:Summary A recessive agagr1 mutation leads to specific defect in sexual agglutinability specifically in agr cells of the yeast Saccharomyces cerevisiae. The cryptopleurine resistance gene cryR 1, closely linked to the mating type locus, was used to select agr/agr strains which emerged from agr/agr strains by mitotic nonreciprocal recombination, to genetically analyse agagr1, since agagr1 is expressed only in agr mating type. The agagr1 gene was found to be linked to the centromere tightly, to met3 at 4.4 cM, and to ilv3 at 12 cM on chromosome X. Sexual agglutinability of agr cells was shown to be dependent on the dose of the AGagr1 gene, using agr/agr isogenic strains carrying AGagr1/AGagr1, AGagr1/agagr1 or agagr1/agagr1. The sst2-1 mutation did not suppress the agagr1 mutation. Based on these results, function of the AGagr1 gene is discussed.Abbreviations cM centimorgan - FDS first division segregation - NPD nonparental ditype - PD parental ditype - SDS second division segregation - TT tetratype
Keywords:agagr1 Mutant" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">1 Mutant  Agglutination  Gene dose effect  Mapping  Saccharomyces cerevisiae
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