Genetic characterization of an α-specific gene responsible for sexual agglutinability in Saccharomyces cerevisiae: mapping and gene dose effect |
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Authors: | Katsunori Suzuki Naohiko Yanagishima |
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Institution: | (1) Biological Institute, Faculty of Science, Nagoya University, 464 Chikusa-ku, Nagoya, Japan |
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Abstract: | Summary A recessive ag 1 mutation leads to specific defect in sexual agglutinability specifically in cells of the yeast Saccharomyces cerevisiae. The cryptopleurine resistance gene cryR
1, closely linked to the mating type locus, was used to select / strains which emerged from / strains by mitotic nonreciprocal recombination, to genetically analyse ag 1, since ag 1 is expressed only in mating type. The ag 1 gene was found to be linked to the centromere tightly, to met3 at 4.4 cM, and to ilv3 at 12 cM on chromosome X. Sexual agglutinability of cells was shown to be dependent on the dose of the AG 1 gene, using / isogenic strains carrying AG 1/AG 1, AG 1/ag 1 or ag 1/ag 1. The sst2-1 mutation did not suppress the ag 1 mutation. Based on these results, function of the AG 1 gene is discussed.Abbreviations cM
centimorgan
- FDS
first division segregation
- NPD
nonparental ditype
- PD
parental ditype
- SDS
second division segregation
- TT
tetratype |
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Keywords: | ag 1 Mutant" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">1 Mutant Agglutination Gene dose effect Mapping Saccharomyces cerevisiae |
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