星点设计-效应面法优化O-羧甲基壳聚糖复凝止血海绵的处方

目的：优化O-羧甲基壳聚糖（O-CMC）复凝止血海绵处方并考察其止血效果。方法：建立兔耳动静脉创伤出血模型,以止血时间及出血量为指标,以水溶性O-CMC为载体,戊二醛为交联剂,加入凝血酶作为复凝血剂,采用星点设计-效应面法优化O-CMC复凝止血海绵处方。结果：O-CMC复凝止血海绵的最优处方为：O-CMC 2.49 mg·ml~（-1）,凝血酶49.87 IU·ml~（-1）,戊二醛0.49 mg·ml~（-1）。其止血效果明显优于O-CMC单方止血海绵和市售明胶海绵。结论：O-CMC复凝止血海绵具有良好的止血效果。

Formula Optimization of O-carboxymethyl Chitosan Multi-hemostatic Sponges by Central Composite De- sign-Response Surface Methodology

Objective： To optimize the tbrmula of O-carboxymethyl chitosan multi-hemostatic sponges and observe the hemostasls efficacy. Method： The model of ear vein and artery traumatic haemorrhage in Japanese white rabbits was established. The hemostasis time and bleeding amount as the indices, the formula of O-carboxymethyl chitosan multi-hemostatic sponges was optimized by central composite design-response surface methodology with soluble O-carboxymethyl chitosan as the carrier, glutaraldehyde as the cross-link- ing agent and thrombin as the multi-coagulant. Result： The optimized concentration of 0-carboxymethyl chitosan, thrombin and glutar- aldehyde was 2.49 mg~ ml - 1,49.87 IU ~ ml - l and 0.49 mg~ ml - 1, respectively. The hemostasis effect of the optimized formula was much better than that of the marketing gelatin sponges. Conclusion： 0-carboxymethyl chitosan muhi-hemostatic sponges show signifi- cant hemostasis efficacy.