| UPLC同时测定野菊花中蒙花苷和绿原酸含量 |
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| 引用本文： | 韩永成,龚海燕,刘伟,陈宁,崔永霞,吴明侠,王丽果,茹凡书.UPLC同时测定野菊花中蒙花苷和绿原酸含量[J].中国实验方剂学杂志,2013,19(20):88-90. |
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| 作者姓名： | 韩永成 龚海燕 刘伟 陈宁 崔永霞 吴明侠 王丽果 茹凡书 |
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| 作者单位： | 河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008;河南中医学院分析测试中心, 郑州 450008 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目(2010A360016) |
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| 摘 要： | 目的: 建立UPLC同时测定不同产地野菊花中蒙花苷和绿原酸含量的分析方法。方法: 采用超高效液相色谱系统, C18 色谱柱(2.1 mm×50 mm,1.8 μm),流动相乙腈-0.1%磷酸水,以0.2 mL·min-1的流速进行梯度洗脱,检测波长326 nm,柱温25℃。结果: 蒙花苷在3.0~120.0 mg·L-1线性关系良好(r=1.000 0),平均回收率99.43%,RSD 1.54%(n=6);绿原酸在0.48~19.2 mg·L-1线性关系良好(r=1.000 0),平均回收率98.67%,RSD 2.13%(n=6)。结论: UPLC法分析速度快,重复性好,结果准确且高效、简便,可用于野菊花中蒙花苷和绿原酸含量的测定。
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| 关 键 词： | 野菊花 超高效液相色谱 蒙花苷 绿原酸 |
| 收稿时间： | 2/1/2013 12:00:00 AM |
Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC |
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| HAN Yong-cheng,GONG Hai-yan,LIU Wei,CHEN Ning,CUI Yong-xi,WU Ming-xi,WANG Li-guo and RU Fan-shu.Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC[J].China Journal of Experimental Traditional Medical Formulae,2013,19(20):88-90. |
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| Authors: | HAN Yong-cheng GONG Hai-yan LIU Wei CHEN Ning CUI Yong-xi WU Ming-xi WANG Li-guo and RU Fan-shu |
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| Institution: | Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China |
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| Abstract: | |
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| Keywords: | Chrysanthemi Indici Flos UPLC buddleoside chlorogenic acid |
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