枳苓六妙口服液对疲劳雄性大鼠血睾酮及相关激素的影响

目的：观察枳苓六妙口服液对疲劳雄性大鼠作用。方法：采用高（360 mg·kg~（-1））、中（118.3 mg·kg~（-1））、低（59.2mg·kg~（-1））不同剂量枳苓六妙口服液给正常wistar雄性大鼠连续灌胃6周,在给药2周、4周、6周后三个时间段,测定其力竭游泳时间和负重运动后雄性大鼠睾酮（T）、皮质酮（C）、促卵泡生成素（FSH）、促黄体生成素（LH）、雌二醇（E_2）和促肾上腺皮质激素（ACTH）的浓度。结果：与对照组比较,枳苓六妙口服液高、中剂量组在3周后均能明显延长雄性大鼠负重游泳时间（P〈0.05或0.01）;6周后,各组均能明显提高雄性大鼠血T浓度和LH浓度（P〈0.05）;高、中剂量组均能显著降低血中C水平（P〈0.05）;6周后,高剂量组能提高雄性大鼠FSH的浓度,差异有统计学意义（P〈0.05）;各剂量组对ACTH和E_2没有明显影响（P〉0.05）。结论：枳苓六妙口服液中、高剂量组具有显著的抗疲劳作用,血清睾酮水平降低可能是疲劳发病机制的重要环节。

Influence of Zhiling Liumiao Oral Solutions on Testosterone and Correlated Hormones in Fatigue Male Rats

Objective ： To research the influence of Zhiling Liumiao oral solutions on testosterone and correlated hormone in fatigue male rats. Method： Zhiling Liumiao oral solutions were administered for 6 weeks in the experimental groups at low （59.2 mg · kg-! ） , medium （118.3 mg · kg-1） and high （360 mg · kg-1） dosage, respectively. The influence of Zhiling Liumiao oral solutions on testosterone and correlated hormones was studied by swimming time and the activity of testosterone （T）, corticosterone （C）, follicle - stimulating hormone （FSH） , luteinizing hormone （LH） , estradiol （ E2 ） and adrenocorticotropic hormone （ACTH）. Result ： Com- pared with the control group, Zhiling Liumiao oral solutions with medium and high dosage could obviously prolong the loading swimming time after 3 weeks （ P 〈 0.05 or P 〈 0.01 ）, increase the T and LH level after 6 weeks （ P 〈 0.05 ） and decrease the C content （ P 〈 0.05 ） , and the high dosage could enhance the FSH activity after 6 weeks （ P 〈 0.05 ）. No significant ACTH and E2 expression was found among those groups （P 〉 0.05 ）. Conclusion： Zhiling Liumiao oral solutions show anti-fatigue effect, especially with the medi- um and high dosage, and the decreased T level may be the mechanism.