肺腺癌中LDH-V、AKT1及Glut1的表达及其与18氟-2-脱氧葡萄糖摄取的相关性研究

目的：探讨肺腺癌中乳酸脱氢酶-V（lactate dehydrogenase V,LDH-V）、丝氨酸/苏氨酸蛋白激酶1（serine-threonine kinase 1,AKT1）及葡萄糖转运蛋白1（glucose transporter 1,Glut1）的表达及它们与18氟-2-脱氧葡萄糖（18F-FDG）摄取的关系。方法：54名肺腺癌患者,术前1周进行PET-CT显像。采用免疫组织化学SP法对LDH-V、AKT1、Glut1的表达进行半定量分析,并与术前PET-CT所得的18F-FDG最大标准化摄取值（maximal standardized uptake value,SUVmax）进行相关性分析。结果：在肺腺癌中,LDH-V、AKT1及Glut1表达的阳性率为88.89%、88.89%、68.52%,LDH-V表达与AKT1及Glut1表达呈正相关（r分别为0.381和0.270,P〈0.01和0.05）;SUVmax与肿瘤最大直径（maximal diameter,Dmax）呈正相关（r=0.524,P〈0.01）,Dmax〉2 cm患者（n=30）的SUVmax与Glut1表达呈正相关（r=0.407,P〈0.05）;SUVmax与病理分级、LDH-V及AKT1表达无关。结论：LDH-V、AKT1及Glut1在肺腺癌中广泛表达且LDH-V表达与AKT1及Glut1表达密切相关,Glut1在肺腺癌18F-FDG摄取中发挥重要作用。

Expression of LDH-V, AKT1, Glut1 and Their Relationships with the Uptake of 18F-FDG in Lung Adenocarcinoma

Objective：To investigate the expression of lactate dehydrogenase V（LDH-V）,serine-threonine kinase 1（AKT1）,glucose transporter 1（Glut1）and their relationships with the uptake of 18F-FDG in lung adenocarcinoma.Methods：A total of 54 patients with lung adenocarcinoma were involved.All patients were examined by PET-CT one week before the operation.The expression levels of LDH-V,AKT1 and Glut1 were detected by SP immunohistochemistry method and assessed by semiquantitative analysis.Correlation analysis was carried out to evaluate correlations of their expression with the uptake of 18F-FDG.Results：Positive expression of LDH-V,AKT1 and Glut1 were found in 88.89 %,88.89 % and 68.52 % of the 54 patients,respectively.Significantly positive correlations were found between the expression of LDH-V and AKT1（r=0.381,P〈0.01）,as well as between LDH-V and Glut1（r=0.270,P〈0.05）.Maximal standardized uptake value（SUVmax）was positively correlated with maximal diameter（Dmax）,r=0.524,P〈0.01.SUVmax was positively correlated with the expression of Glut1 in patients whose Dmax2 cm（n=30）,r=0.407,P〈0.05.SUVmax had no correlation with pathological grading and the expression of AKT1 or Glut1.Conclusions：LDH-V,AKT1 and Glut1 are widely and obviously expressed in lung adenocarcinoma.The expression of LDH-V was positively correlated with AKT1 and Glut1.Glut1 plays an important role in the uptake of 18FFDG.