补心方对慢性心衰大鼠脑钠肽、肌钙蛋白I及高能磷酸盐的影响

研究补心方对心梗后心衰大鼠心肌脑钠肽(B-type natriuretic peptide,BNP)、肌钙蛋白Ⅰ(troponin I,TNI)与高能磷酸盐水平的影响.方法:大鼠60只,经结扎冠状动脉前降支中上1/3部,制作急性心肌梗死(AMI)模型,手术成功2周后,将存活动物随机分为补心方高、中、低剂量组及曲美他嗪组、模型组、假手术组共6组,均予灌胃给药或蒸馏水8周(曲美他嗪混悬液1.0 mg·kg-1·d-1 ig;补心方高、中、低剂量组依次为15.6,7.8,3.9g·kg-1·d-1).采用酶联免疫吸附法检测药物干预前后静脉血中BNP及TNI水平,高压液相色谱法检测高能磷酸盐含量.结果:对BNP与TNI的影响:治疗前,与假手术组相比,模型组及余各组大鼠BNP,TNI水平显著升高(P＜0.01);补心方干预后,与模型组比较,中药各组大鼠BNP,TNI含量显著下降(P＜0.01).对高能磷酸盐的影响:与假手术组比较,模型组大鼠ATP含量明显降低(P＜0.01).补心方干预后,高剂量组及低剂量组ATP含量均升高(P ＜0.05,P＜0.01).结论:补心方可显著降低心衰大鼠心肌BNP及TNI含量,减轻心肌损伤,改善能量代谢,从而改善慢性心衰大鼠症状,延缓慢性心衰的发展.

Effect of Buxin Decoction on Content of B-type Natriuretic Peptide, Troponin I, Adenosine Triphosphate in Rats with Chronic Heart Failure after Myocardial Infarction

Objective: To observe the effect of Buxin decoction on the content of B-type natriuretic peptide(BNP),troponin I(TNI),adenosine triphosphate(ATP) in rats with heart failure after myocardial infarction. Method: Sixty rats were randomly divided into 6 groups:model group,sham-operation group,trimetazidine(TMZ) group low, middle and high dosage groups of Buxin decoction,10 rats in each group. The acute myocardial infarction (AMI) model was induced by ligation of the left anterior descending artery, oral administration lasted for 8 weeks.The BNP,TNI level was determined by enzyme-linked immuno sorbent assay(ELISA),and the contents of adenosine monophosphate(AMP),adenosine diphosphate(ADP) and ATP were measured by HPLC. Result: Before treatment, compared with the sham-operation group,BNP and TNI level in model and treated group significantly increased (P<0.01). After treatment,compared with model group,BNP and TNI level in Buxin decoction groups significantly decreased (P<0.01). Compared with the sham-operation group, the ATP content in model group was significantly lower (P<0.01). After the intervention of Buxin decoction, the ATP content increased in the high-dose and low dose group(P<0.05, P<0.01). Conclusion: Buxin decoction can reduce the content of BNP and TNI in rats with heart failure after myocardial infarction,and improve energy metabolism.