龙胆掩味前后指纹图谱变化

目的:建立龙胆药材掩味前后的指纹图谱,比较其掩味前后的变化,总结苦味抑制剂对龙胆的掩味规律.方法:采用三氯蔗糖(SL)、单磷酸腺苷(AMP)、阿魏酸钠(SF)3种苦味抑制剂单独或联合应用对龙胆水煎液掩味,并用高效液相色谱法采集掩味前后各样品液的指纹图谱.结果:各平行色谱峰相对保留时间没有显著差异,最大差异仅为2.2％;含AMP组的指纹图谱个别峰的相对峰面积差异较大,最高达93.7％,但龙胆苦苷相对峰面积差异均在4.8％以下,而不含AMP组的指纹图谱中仅1和11号峰变化较大,最大变化达19.0％,16.4％,其他各峰相对峰面积差异均在10％以下.结论:SL,SF对龙胆的成分没有太大的影响,不会影响到龙胆的药物疗效,而AMP对龙胆部分成分有不同程度的影响,但是否影响到龙胆的药效有待进一步的研究.

Changes of Fingerprints of Radix Gentianae before and after Taste Masking

Objective: To establish the HPLC fingerprint of Radix Gentianae before and after taste masking, compare the changes and summary the taste masking regular pattern. Method: Suc-ralose(SL), adenosine monophosphat(AMP) and sodium ferulate(SF) alone or in combination to masking the taste of Radix Gentianae were used, and the fingerprints of every sample were collected before and after taste masking use HPLC. Result: In the relative retention time there was no significant difference between every parallel peak, the maximum difference was just 2.2%;In the relative peak area, some peaks were quit different which contain AMP, the highest one reached to 93.7%, but the relative peak area of gentiopicroside was just under 4.8%, only 1 and 11 peaks in the fingerprints without AMP group had larger changes, the maximum changes reached to 21.4% and 16.4%, the relative peak area of each peak difference was in 10%. Conclusion: Sucralose and sodium ferulate have no impacton the elements and efficacy of Radix Gentianae;AMP has some impact on some elements of Radix Gentianae, but there is needed to do some further research wether AMP will impact the efficacy of Radix Gentianae.