外翻肠囊法研究白芷有效组分对配伍"对药"有效成分肠吸收的影响

目的: 考察白芷有效组分香豆素和挥发油分别对配伍"对药"葛根中葛根素、黄芩中黄芩苷、川芎中阿魏酸及甘草中甘草酸、甘草苷肠吸收的影响,探讨白芷对配伍"对药"有效成分肠吸收的影响规律。方法: 采用大鼠外翻肠囊吸收模型,分别考察白芷有效组分配伍各对药(葛根、黄芩、川芎、甘草)前后,葛根中葛根素、黄芩中黄芩苷、川芎中阿魏酸及甘草中甘草酸、甘草苷在不同肠段的吸收情况。结果: 白芷有效组分能显著促进葛根中葛根素(提高1.70倍)、黄芩中黄芩苷(提高4.74倍)的肠吸收作用;白芷有效组分对川芎中阿魏酸肠吸收无显著影响;白芷有效组分对甘草中甘草苷、甘草酸具有显著的肠吸收抑制作用。结论: 白芷-葛根,白芷-黄芩药对配伍应用产生协同治疗作用,不仅表现为不同活性成分作用于机体的不同靶点,还表现为白芷活性成分促进其配伍"对药"活性成分肠道转运。白芷-川芎、白芷-甘草配伍机制可能与白芷对甘草中活性成分肠吸收的影响无关。

Influence of Active Compounds of Angelicae Dahuricae Radix on the Intestinal Absorption of Active Components of Compatibility of Drug Pairs Using Everted Gut Sac Model

Objective: To investigate the influence of active compounds of Angelicae Dahuricae Radix(coumarin/volatile oil) on the intestinal absorption of puerarin, baicalin, ferelic, liquiritin and glycyrrhizic acid, respectively. To explore the influence law of Angelicae Dahricae Radix on the intestinal absorption of the active compounds of herb-pairs. Method: Everted gut sac was used to study compatible effects of Angelicae Dahuricae Radix and herb-pairs(Puerariae Lobatae Radix, Scutellariae Radix, Chuanxiong Rhizoma, Glycyrrhizae Radix et Rhizoma) on intestinal absorption of puerarin, baicalin, ferelic acid, liquiritin and glycyrrhizic acid. Result: Results showed that Angelicae Dahuricae Radix could enhance the absorption of puerarin(1.7 times ) and baicalin (4.74 times ), and has no effect on the intestinal absorption of ferulic acid, but could significantly restrain the intestinal absorption of liquiritin and glycyrrhizic acid. Conclusion: These results confirmed that synergistic therapeutic effect of compatibility of Angelicae Dahuricae Radix and Puerariae Lobatae Radix, Angelicae Dahuricae Radix and Scutellariae Radix not only lay in the different active ingredients acting on the different target, but also shown as active compounds of Radix Angelicae dahuricae could increasing the intestinal absorption of puerarin and baicalin. The mechanism of Angelicae Dahuricae Radix and Chuangxiong Rhizoma, Angelicae Dahuricae Radix and Glycyrrhizae Radix et Rhizoma may be not related to the influence of Angelicae Dahuricae Radix on the intestinal absorption of ferulic acid, liguiritin and glycyrrhizic acid.