复方双参合剂对小鼠耐缺氧和抗运动疲劳能力的影响

目的：研究复方双参合剂对小鼠耐缺氧和抗疲劳的作用。方法：50只小鼠随机分为空白组（生理盐水10 ml·kg~（-1））、丹参组（丹参提取物0.2 g·kg~（-1））、人参组（人参皂苷0.1 g·kg~（-1））、复方双参组（丹参提取物0.2 g·kg~（-1）＋人参皂苷0.1 g·kg~（-1））和红景天组（阳性对照,红景天提取物0.6 g·kg~（-1））,每组10只。连续灌胃给药20 d后,测定小鼠常压密闭缺氧存活时间、减压缺氧存活率和负重游泳时间,并测定小鼠运动后血清尿素氮含量、肝糖原含量、血乳酸含量和乳酸脱氢酶活性。结果：与空白组相比,复方双参组小鼠常压密闭缺氧存活时间显著延长,延长率为45.95%（P〈0.01）;小鼠运动后血清尿素氮含量及乳酸堆积显著减少（P〈0.01）,肝糖原含量及乳酸脱氢酶活性显著增加（P〈0.01）,其作用强于红景天组。减压缺氧条件下,给药组存活率较空白组显著提高（P〈0.05或0.01）。红景天组与复方双参组与其他给药组相比,小鼠负重游泳时间显著延长（P〈0.05或0.01）。结论：复方双参合剂能显著增强小鼠耐缺氧和抗疲劳的能力。

Effects of Compound Shuangshen Mixtures on Anti-hypoxia and anti-fatigue in Mice

Objective ： To study the anti-hypoxia and anti-fatigue effects of compound Shuangshen mixtures in mice. Method ： To- tally 50 mice were randomly divided into 5 groups, the blank control group （ physiological saline, 10 ml · kg-1 ） , Danshen group （ sal- via extract, 0.2 g · kg - 1 ）, Renshen group （ ginsenoside, 0.1 g · kg - 1 ） , the compound Shuangshen group （ salvia extract 0.2 g · kg-l plus ginsenoside 0.1 g · kg-1） and rhodiola group （the positive control group, rhodiola extract, 0.6 g · kg-l）. Mice were ad- ministrated by garage once daily for 20 days. Then the endurance time with hypoxia at normal pressure, the survival ratio with hypoxia at decompression and the loaded-swimming time of mice were measured. The contents of serum urea nitrogen and hepatic glycogen, lac- tic acid level and lactate dehydrogenase activity were tested after the swimming. Result： Compound Shuangshen mixtures could signifi- cantly prolong the endurance time with hypoxia at normal pressure with the prolongation rate of 45.95% （P 〈 0.01 ）. The survival ratio of the administration groups was increased significantly compared with that of the blank control group with hypoxia at decompression （ P 〈 0.05 or 0.01 ）. Compound Shuangshen mixtures could prolong significantly the loaded-swimming time than Danshen group and Ren- shen group（ P 〈 0.05 ）. Compound Shuangshen mixtures could decrease significantly serum urea nitrogen and the content of lactic acid （P 〈 0.01 ）, increase significantly hepatic glycogen reserves and lactate dehydrogenase activity （P 〈 0.01 ） compared with the blank control group after the swimming. Compound Shuangshen mixtures had better effectiveness compared with rhodiola group. Conclusion： Compound Shuangshen mixtures can siguificantlv improve the ability of anti-hypoxia and anti-fatigue in mice.