ACE基因rs4353,rs4461142和rs8066114位点多态性与心肌梗死的相关性分析

目的 探讨陕西地区汉族人群中,血管紧张素转换酶(ACE)基因多态性与心肌梗死(MI)的相关性。方法 选择2013年1月～2017年1月住院治疗的346例急性心肌梗死(AMI)患者纳入研究记为MI组,另选择300例健康志愿者作为对照组,采用ELISA法检测血清ACE水平,采用聚合酶链反应-限制性片段长度多态性法检测ACE基因rs4353,rs4461142和rs8066114多态性,并收集患者临床资料进行分组及统计学分析。结果 MI组与对照组比较,两组血清ACE水平34.7±13.8U/L vs 30.6±12.0 U/L,差异具有统计学意义(t=3.998,P<0.05)。对于对照组患者,ACE基因rs4353和rs4461142位点不同基因型血清ACE水平差异具有统计学意义(F=40.860,3.382,均P<0.05); rs8066114位点不同基因型血清ACE水平差异无统计学意义(F=0.176,P>0.05)。MI组和对照组比较,ACE基因rs4353位点基因型和等位基因分布频率差异具有统计学意义(χ²=8.146,7.585,均P<0.05),两组rs4461142和rs8066114位点基因型和等位基因分布频率差异均无统计学意义(χ²=2.263～4.016,均P>0.05)。多因素Logistic回归分析结果显示ACE基因rs4353位点A等位基因是MI危险因素,可显著增加MI发生风险(OR=1.620,95%CI 1.217～4.723,P=0.035)。结论 ACE基因rs4353位点多态性可能与MI易感性相关。

Association between Angiotensin Converting Enzyme Gene rs4353, rs4461142 and rs8066114 Polymorphism and Myocardial Infarction

Objective To investigate the genetic association between angiotensin-converting enzyme(ACE)gene polymorphism and myocardialinfarction(MI)stroke in the Han descent population of Shaanxi Province.Methods 346 patients with acute myocardial infarction(AMI)from January 2013 to January 2017 were recruited to participate in the study as theMI group.Meanwhile,300 healthy volunteers without MI were recruited to participate in the study as the control group.Serum ACE was detected by ELISA technique.Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism for the ACE gene rs4353,rs4461142 and rs8066114 polymorphism,and collected other clinical data for statistical analysis.Results There was significant difference in serum ACE level between MI group and control group(34.7±13.8 U/L vs 30.6±12.0 U/L,t=3.998,P<0.05).For controls,there were significant difference in serum ACE level among different genotype of rs4353 and rs 4461142(F=40.860 and 3.382,all P<0.05).However,there was no significant difference in serum ACE level among different genotype of rs8066114(F=0.176,P>0.05).There were significant difference in the genotype and allele frequencies of ACE gene rs4353 between MI group and control group(χ²=8.146 and 7.585,all P<0.05).However,genotype and allele frequencies of rs4461142 and rs8066114 did not differ significantly between MI group and control group(χ²=2.263～4.016,all P>0.05).Multivariate Logistic regression analysis showed that polymorphism of ACEgene rs4353 was risk factor for MI(OR=1.620,95%CI 1.217～4.723,P=0.035).Conclusion ACE gene rs4353 polymorphism may closely related to MI.