| 紫外分光光度法测定蛹虫草菌丝体中硒的含量 |
| |
| 引用本文： | 张月,钟鸣,林景卫,李浩戈,马慧.紫外分光光度法测定蛹虫草菌丝体中硒的含量[J].微量元素与健康研究,2010,27(3):21-23. |
| |
| 作者姓名： | 张月 钟鸣 林景卫 李浩戈 马慧 |
| |
| 作者单位： | 沈阳农业大学辽宁省生物技术重点实验室,沈阳,110161 |
| |
| 基金项目： | 辽宁省农业生物技术重点实验室专项资金资助(2009) |
| |
| 摘 要： | 采用紫外分光光度法,以邻苯二胺盐酸盐做显色剂,检测波长为335nm,测定蛹虫草菌丝体中硒元素的含量。结果表明,吸收度与浓度之间呈良好线性关系(r2=0.9998),蛹虫草菌丝体干重中的硒元素含量为7.69μg/g,RSD为0.037%(n=7),平均回收率97.24%,方法操作简便、快速、灵敏度好。
|
| 关 键 词： | 蛹虫草 硒含量 紫外分光光度法 灵敏度 |
Ultraviolet Spectrophotometry Determine Selenium Content in Cordyceps militaris mycelium |
| |
| ZHANG Yue,ZHONG Ming,LIN Jing-wei,LI Hao-ge,MA Hui.Ultraviolet Spectrophotometry Determine Selenium Content in Cordyceps militaris mycelium[J].Studies of Trace Elements and Health,2010,27(3):21-23. |
| |
| Authors: | ZHANG Yue ZHONG Ming LIN Jing-wei LI Hao-ge MA Hui |
| |
| Institution: | (Shenyang Agriculture University Liaoning Key Laboratory of Agriculture Biotechnology,Shenyang 110161,China) |
| |
| Abstract: | Ultraviolet spectrophotometry was used to determine content of trace selenium in Cordyceps militaris mycelium with o-phenylenediamin hydrochloride as apparent color agent,and the detection wavelength was 335 nm.The results show that there was a good linear relationship between absorbency and concentration（r^2=0.9998）.The trace element selenium content of dry weight of Cordyceps militaris mycelia was 7.69 μg/g,and the average recovery was 97.24 % with RSD 0.037%（n=7）.This method was simple,quick and sensitive. |
| |
| Keywords: | Cordyceps militaris selenium content ultraviolet spectrophotometry sensitive |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
