L-单甲基-精氨酸对颞下颌关节骨关节病治疗作用的实验研究

目的　研究一氧化氮合酶抑制剂L 单甲基 精氨酸对山羊实验性颞下颌关节骨关节病的治疗作用及可能机制。方法　通过双侧颞下颌关节上腔内注射胶原酶建立骨关节病模型 ,从注射胶原酶第 4周起 ,在左侧关节上腔内每隔 3d注射 0 5 %L 单甲基 精氨酸 0 5ml,共 7次 ,右侧同期注射生理盐水 0 5ml。注射胶原酶后 1 2周处死所有动物 ,取双侧关节标本作组织学检查并根据改良Mankin骨关节病评价标准评分。结果　评分结果 :L 单甲基 精氨酸侧为 3 83 ,而对照侧为 6 33 ,两者比较差异有高度显著性 (P <0 0 1 )。结论　L 单甲基 精氨酸关节腔内注射对山羊实验性颞下颌关节骨关节病的发展有抑制作用 ,其作用机制可能与L 单甲基 精氨酸抑制一氧化氮合酶在关节局部产生过多的一氧化氮有关

The effects of L-NMMA on experimental temporomandibular joint osteoarthrosis in goats

OBJECTIVE: To investigate the effects of repeated injections of L-NMMA on a goat model with osteoarthrotic temporomandibular joint (TMJ) disease. METHODS: Eight goats were selected in this study. Bilateral TMJ osteoarthrosis (OA) was induced by injecting 0.5% collagenase. L-NMMA was injected into one side of TMJs at 4 weeks after collagenase injection (one time every three days). Another joint as control was simultaneously injected using 0.9% saline solution. All goats were killed at 12 weeks after collagenase injection. The TMJ specimens were harvested and processed for histological examination. Modified Mankin's grading score system was used for evaluating changes in the TMJ. RESULTS: The control side of TMJs showed severe osteoarhrotic changes in the condyle whereas the L-NMMA-treated TMJs showed less degenerative alterations. The histologic score was 3.83 in the L-NMMA treated side, and 6.33 in the control. There was a significant difference in osteoarthrotic changes between the L-NMMA-treated and control TMJs (P < 0.01). CONCLUSIONS: Repeated intra-articular injection of L-NMMA into TMJ may play a role in inhibiting TMJOA progression.