| 用PCR法分析中国汉族群体D17S30位点遗传多态性 |
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| 引用本文： | 陈林 孙光云. 用PCR法分析中国汉族群体D17S30位点遗传多态性[J]. 中华医学遗传学杂志, 1995, 12(2): 85-88 |
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| 作者姓名： | 陈林 孙光云 |
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| 作者单位： | 华西医科大学法医系血清学教研室 |
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| 摘 要： | 用聚合酶链式反应对中国汉族群体１２２名无关个体Ｄ１７Ｓ３０位点扩增片段长度多态性进行分析研究，发现１０个等位基因，片段大小为１６８－７９８ｂｐ，基因频率为０．００８２－０．２５８２，杂合性为７８％。５５种可能基因型中发现了２７种，对基因型的观察值和期望值进行Ｘ＾２检验，符合Ｈａｒｄｙ－Ｗｅｉｂｅｒｇ平衡定律。
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| 关 键 词： | D17S30位点 汉族 聚合酶链反应 基因 多态性 |
POLYMORPHIC ANALYSIS OF THE D17S30 LOCUS IN A CHINESE HAN POPULATION BY THE POLYMERASE CHAIN REACTION |
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| Chen Lin,Sun Guangyun, Wu Meiyun.. POLYMORPHIC ANALYSIS OF THE D17S30 LOCUS IN A CHINESE HAN POPULATION BY THE POLYMERASE CHAIN REACTION[J]. Chinese journal of medical genetics, 1995, 12(2): 85-88 |
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| Authors: | Chen Lin Sun Guangyun Wu Meiyun. |
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| Abstract: | A genetic locus (D17S30) that contains a variable number of tandem repeat (VNTR) has been successfully amplified from a very small quantity of genomic deoxyribonucleic acid (DNA) by the polymerase chain reaction (PCR). PCR products were separated on agarose gel electrophoresis and visualized by ethidium bromide staining; the amplified-fragment-length polymorphism (AmpFLP) was shown. Analysis of 122 normal individuals of Han population revealed 10 alleles (differing in copy number of a 70 bp repeat unit) ranging from 168 to 798 bp in size. The allele frequencies were 0.0082 to 0. 2582;The heterozygosity of D17S30 locus was 78%, and the power of discrimination was 0.9384. Twenty seven different phenotyes were observed. The observed nurnber of genotypes were in good agreement with the expected number under the Hardy-Weinberg equilibrium (X2=11. 76,df=8,0.10
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| Keywords: | D17S30 locus Variable number tandem repeat Polymerase chain reaction Amplified-fragment-length polymorphism |
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