| 用PCR技术产前诊断人巨细胞病毒感染 |
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| 引用本文： | 孙永玉,陶幼慈.用PCR技术产前诊断人巨细胞病毒感染[J].中华医学遗传学杂志,1995,12(5):279-281,T018. |
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| 作者姓名： | 孙永玉 陶幼慈 |
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| 作者单位： | 同济医科大学附属协和医院妇产科遗传室 |
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| 摘 要： | 用ＥＬＩＳＡ法筛选早孕妇女血人巨细胞病毒ＩｇＭ（ＨＣＭＶ－ＩｇＭ），在孕１２￣２０周用聚合酶链式反应（ＰＣＲ）技术检测其羊水中ＨＣＭＶ－ＤＮＡ，对胎儿巨细胞病毒感染作出产前诊断。在３８４例孕妇中，筛选出血ＨＣＭＶ－ＩｇＭ阳性２６例为实验组，其羊水ＨＣＭＶ－ＤＮＡ检出率为３４．６２％；从２５８例ＨＣＭＶ－ＩｇＭ阴性中选出２０例为对照组，其羊水ＨＣＭＶ－ＤＮＡ检出率为１０．０％，两组比较有显著性差异（
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| 关 键 词： | 巨细胞病毒感染 胎前诊断 聚合酶链式反应 |
PRENATAL DIAGNOSIS OF HUMAN CYTOMEGALOVIRUS INFECTION BY POLYMERASE CHAIN REACTION |
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| Sun Yongyu,Tal Youci,Jiang Xiaowei..PRENATAL DIAGNOSIS OF HUMAN CYTOMEGALOVIRUS INFECTION BY POLYMERASE CHAIN REACTION[J].Chinese Journal of Medical Genetics,1995,12(5):279-281,T018. |
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| Authors: | Sun Yongyu Tal Youci Jiang Xiaowei |
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| Abstract: | Human Cytomegalovirus IgM (HCMV-IgM)in blood of 384 pregnant women in 12-20 weeks of gestation was detected by ELISA, and 26 pregnant women of them were positive for HCMV-IgM in blood. The amniotic fluid of these 26 cases(as study group) and 20 negative cases(selected as controls) was collected and HCMV-DNA was detected by polymerase chain reaction (PCR) to diagnosis fetal HCMV infection before birth. The results showed that 9 of the 26 cases and 2 of the 20 were positive for HCMV-DNA, constituting a total positive rate of 23.91% (11/46). Umbilical cord blood was detected by PCR in 11 positive cases;all of them were positive for HCMV-DNA PCR. |
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| Keywords: | Polymerase chain reaction Cytomegalovirus Prenatal diagnosis |
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