烧伤早期大鼠回肠肌间神经丛内氮能神经和Cajal间质细胞的变化

目的了解烧伤早期大鼠肠功能障碍时肌间神经丛的形态学改变，并探讨肠功能异常的机制。方法20只大鼠随机分成对照组（n=10）和烧伤组（n=10），建立30％TBSA深Ⅱ度烧伤大鼠模型，测定肠传输速率。用酶组织化学方法观察回肠肌间神经丛内氮能神经的组织学改变，并对两组大鼠回肠肌间神经丛内一氧化氮合酶（NOS）阳性神经元、神经节和Cajal间质细胞（ICC）的密度进行测定。结果烧伤组大鼠肠传输速率明显延迟，回肠肌间神经丛内NOS阳性神经元和ICC数量均明显减少（P〈0．01），同时伴有神经纤维部分断裂，未能形成完整的神经纤维网。结论烧伤大鼠回肠肌间神经丛氮能神经和ICC的改变可能是烧伤引起肠功能障碍的原因。

Changes in nitriergic nerves and interstitial cell of Cajal in intestinal myenteric plexus of scalded rat during early post-burn stage

Objective To study the changes in nitriergic nerves and interstitial cell of Cajal (ICC) in intestinal myenteric plexus of scalded rats during early post-burn stage. Methods 20 rats were randomly divided into scald group (n=10) and control group (n=10). A rat model of 30% TBSA with II degree scald was reproduced in the scald group. The rate of enteric transit was measured, and the histological changes in nitriergic nerves in intestinal myenteric plexus of scalded rat were observed as well. The densities of neurons and ganglions with positive nitric oxide synthetase (NOS) as well as ICC in duodenal myenteric plexus were assessed for both groups. Results In the rats in scalded group, the rate of enteric transit was prolonged, and the number of nitriergic nerves and ICC in myenteric plexus of rat duodenum were significantly decreased (P<0.01) compared with control group. Conclusion The changes in nitriergic nerves and ICC in myenteric plexus of rat duodenum might be closely related to intestinal dysfunction during early post-burn stage.