| 荧光PCR技术检测HBV—DNA的初步研究 |
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| 引用本文： | 周斌,胡志东,阎欢,钟述猷.荧光PCR技术检测HBV—DNA的初步研究[J].天津医药,2001,29(9):534-535. |
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| 作者姓名： | 周斌 胡志东 阎欢 钟述猷 |
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| 作者单位： | 天津医科大学总医院 |
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| 摘 要： | 目的：研究荧光PCR技术在临床的应用价值。方法：选择90份HBV－ELISA阳性标志物血清进行HBV－DNA的检测，从血清中提取DNA，运用iCycler-PCR软件进行荧光PCR扩增，对结果进行分析。结果：HBsAg-HBeAg-HBcAb组阳性率为96．7％，HBsAg-HBeAb-HBcAb组阳性率80％，HBeAg-HBcAb组阳性率55％。结论：荧光PCR技术是一种良好的检测HBV－DNA的技术，但尚不能作为乙型肝炎治愈与否的诊断标准。
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| 关 键 词： | 聚合酶链反应 乙型肝炎病毒 DNA 乙型肝炎 |
Pilot Study on Detection of HBV-DNA by FQ-PCR |
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| ZHOU Bin,HU Zhidong,YAN Huan,et al.Pilot Study on Detection of HBV-DNA by FQ-PCR[J].Tianjin Medical Journal,2001,29(9):534-535. |
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| Authors: | ZHOU Bin HU Zhidong YAN Huan |
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| Institution: | ZHOU Bin,HU Zhidong,YAN Huan,et al Department of Microbiology,Tianjin Medical University Hospital 300052 |
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| Abstract: | To study the clinical significance of the technique of quantitative fluorescence-polymerase chain reaction (FQ-PCR). Methods: Ninety serous samples were selected from the patients with positive HBV results by ELISA and amplified by FQ-PCR. Their results were analyzed with the software of iCycler-PCR. Results: The percent of HBsAg-HBeAg-HBcAb having positive results was 96.70 % , while the positive rate of HBsAg-HBeAg-HBcAb was 80.00 % , and 55.00 % of the patients had a positive sign of HBsAg-HBcAb. Conclusion: FQ-PCR is a good quantitative method to detect HBV-DNA, but it is not a diagnostic standard to identify whether the hepatitis B is cured or not. |
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| Keywords: | polymerase chain reaction hepatitis B virus DNA viral |
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