| siRNA沉默人乳腺癌细胞株CXCR4基因的实验研究 |
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| 引用本文： | 厉红元,任国胜,谭金详.siRNA沉默人乳腺癌细胞株CXCR4基因的实验研究[J].第三军医大学学报,2007,29(8):728-730. |
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| 作者姓名： | 厉红元 任国胜 谭金详 |
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| 作者单位： | 重庆医科大学附属第一医院普通外科,重庆,400016 |
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| 摘 要： | 目的 探讨shRNA- CXCR4对人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435s细胞CXCR4基因表达及蛋白表达的影响.方法 通过shRNA-CXCR4沉默CXCR4基因后,RT-PCR、Western blotting检测3株人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435s的CXCR4 mRNA及其蛋白的表达.结果 shRNA-CXCR4作用于人乳腺癌细胞株MCF-7、MDA-MB-231、MDA-MB-435s后能明显抑制其CXCR4基因的mRNA及蛋白表达水平(P＜0.05).结论 shRNA-CXCR4能特异性抑制乳腺癌细胞的CXCR4 mRNA及其蛋白的表达.
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| 关 键 词： | 乳腺癌 CXCR4基因 |
| 文章编号： | 1000-5404（2007）08-0728-03 |
| 修稿时间： | 2007-03-09 |
Silencing breast cancer cell CXCR4 gene in vitro |
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| LI Hong-yuan,REN Guo-sheng,TAN Jin-xiang.Silencing breast cancer cell CXCR4 gene in vitro[J].Acta Academiae Medicinae Militaris Tertiae,2007,29(8):728-730. |
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| Authors: | LI Hong-yuan REN Guo-sheng TAN Jin-xiang |
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| Institution: | Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | Objective To study whether shRNA-CXCR4 could inhibit the CXCR4 gene expression in MCF-7,MDA-MB-231 and MDA-MB-435s breast cancer cells.Methods After the knockdown of CXCR4 by shRNA,the CXCR4 mRNA expression by RT-PCR and the CXCR4 protein expression by Western blotting in breast cancer cells were examined.Results The CXCR4 mRNA expression and its protein expression were decreased significantly in MCF-7,MDA-MB-231 and MDA-MB-435s breast cancer cells by shRNA-CXCR4(P<0.05).Conclusion Silencing CXCR4 could inhibit the breast cancer cell CXCR4 mRNA and its protein expression. |
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| Keywords: | shRNA |
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