PTEN和P-ERK蛋白在皮肤鳞状细胞癌中的表达和意义

目的:探讨PTEN和P-ERK蛋白在皮肤鳞状细胞癌中的表达及意义.方法:采用免疫组化SP法观察34例皮肤鳞癌组织和15例正常皮肤组织石蜡标本中PTEN蛋白和P-ERK蛋白的表达情况.结果:皮肤鳞状细胞癌(SCC)组织中PTEN蛋白阳性表达率(41.18%)明显低于正常皮肤组织(100%)(P<0.05);P-ERK蛋白在SCC中的阳性表达率(76.47%)明显高于正常皮肤组织(20.00%)(P(0.05):SCC中低分化组的PTEN和P-ERK蛋白的阳性表达率分别低于和高于高分化组(P<0.05).SCC中PTEN和P-ERK蛋白表达之间呈明显的负相关(P<0.01).结论:PTEN蛋白的低表达或失表达,不能有效抑制Ras/Raf/MEK/ERK信号通路的异常激活,可能使细胞恶性转化和增殖.

The expression and significance of PTEN and P-ERK protein in cutaneous squamous cell carcinoma

Objective To investigate the expression and significance of PTEN and P-ERK protein in cutaneous squamous cell carcinoma.Methods Expression of PTEN and P-ERK protein was detected in 34 SCC and 15 normal skin specimens by SP immunohistochemical technique.Results The positive rate of PTEN protein was significantly lower in SCC(41.18%) than that in normal skin(100%)(P<0.05);The positive rate of P-ERK was significantly higher in SCC(76.47%) than that in normal skin(20.00%)(P<0.05);The positive rate of PTEN and P-ERK protein in the low differentiation group were lower and higher than them in the high differentiation group(P<0.05),A significant negative relationship was observed between the expression of PTEN and P-ERK protein in SCC(P<0.01).Conclusions The decrease or deletion of PTEN protein expression may not be able to effectively inhibit the over-activation of Ras/Raf / MEK/ERK signaling pathway,which will result in the malignant transformation and proliferation.