首页 | 本学科首页   官方微博 | 高级检索  
     

水痘-带状疱疹病毒临床分离株基因型别分析以及与Oka疫苗株的区别
引用本文:刘静静,王明丽,甘霖,杨森,陈敬贤. 水痘-带状疱疹病毒临床分离株基因型别分析以及与Oka疫苗株的区别[J]. 中华微生物学和免疫学杂志, 2009, 29(3). DOI: 10.3760/cma.j.issn.0254-5101.2009.03.010
作者姓名:刘静静  王明丽  甘霖  杨森  陈敬贤
作者单位:1. 安徽医科大学微生物学教研室,合肥,230032
2. 安徽医科大学皮肤性病研究所,230032
3. 230032,合肥,安徽医科大学微生物学教研室;NY,10032,纽约,哥伦比亚大学病理与细胞生物学教研室
摘    要:目的 运用分子生物学方法 对从水痘或带状疱疹患者皮肤疱疹液中分离得到的水痘-带状疱疹(VZV)株进行基闪型研究,并区分感染是南野牛株还是由Oka疫苗株引起的.方法 从水痘或带状疱疹患者的皮肤水疱液中分离VZV,然后利用聚合酶链反应和限制性片段长度多态性分析对病毒株的ORF38、54、62和R5可变区基因进行分析.结果 在所分离的19株VZV中,存在PstⅠ+ Bgl Ⅰ+ R5A和Pst Ⅰ+ Bgl Ⅰ+RSB两种基因型,其中Pst Ⅰ+ Bgl Ⅰ+ R5A占52.7%,Pst Ⅰ+ Bgl Ⅰ+R5B占47.3%,没有发现与Oka疫苗株相同的基因型.结论 本研究中所分离的VZV毒株均系野生株,它们的基因型与欧洲、美国、日本的VZV分离株均不相同.利用病毒基因组中ORF38和ORF62区域的单一核苷酸多态性,能够区分疫苗株和野毒株.

关 键 词:水痘-带状疱疹病毒  基因分型  Oka疫苗株  野毒株

Genotyping of wild-type strains of varicella-zoster virus and their differentiation from Oka vaccine strain
LIU Jing-jing,WANG Ming-li,GAN Lin,YANG Sen,CHEN Jin-xian. Genotyping of wild-type strains of varicella-zoster virus and their differentiation from Oka vaccine strain[J]. Chinese Journal of Microbiology and Immunology, 2009, 29(3). DOI: 10.3760/cma.j.issn.0254-5101.2009.03.010
Authors:LIU Jing-jing  WANG Ming-li  GAN Lin  YANG Sen  CHEN Jin-xian
Abstract:Objective To analyze the genotype of varicella-zoster virus (VZV) strains isolated from patients with chickenpox or zoster and to differentiate them from Oka vaccine strain by molecular analysis. Methods In the present study, single nucleotide polymorphisms(SNP) based VZV genotypes were analyzed in 19 VZV isolates using the polymerase chain reaction and restriction fragment length polymorphisms analysis of DNA fragments of the open reading frames 38, 54, 62, and the 1t5 repeat region. Results The genotypes of 19 VZV isolates including two different groups with 52.7% of Pst Ⅰ+ Bgl Ⅰ+ R5A and 47.3% of Pst Ⅰ+ Bgl Ⅰ+ R5B, which is very different from those found in North America, Europe and Japan. All the Chinese isolates are wild-type strains with ORF62 Sma Ⅰ-. No Oka vaccine strains were revealed among the isolates. Conclusion Chinese VZV strains reported in this study showed different molecular characteristics from those circulating in Europe, North America and Japan. The SNPs in ORF62 and ORF38 may be used to distinguish VZV wild-type strains and vaccine strain in clinical isolates in China.
Keywords:Varicella-zoster virus  Genotyping  Oka strains  Wild-type strains
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号