浅表性胃炎、胃粘膜不典型增生及胃癌组织中COX-1、COX-2、iNOS表达的意义

目的：研究ＣＯＸ－１、ＣＯＸ－２、ｉＮＯＳ于浅表性胃炎、胃粘膜不典型增生及胃癌中表达的意义，探讨ＣＯＸ－２，ｉＮＯＳ与胃癌、幽门螺杆菌（Ｈｐ）感染的关系。方法应用多克隆抗体ＣＯＸ－１，ＣＯＸ－２、ｉＮＯＳ免疫组化方法检测３３例浅表性胃炎、３０例胃粘膜不典型增生、２９胃癌石蜡包埋组织中的ＣＯＸ－１、ＣＯＸ－２、ｉＮＯＳ表达情况及Ｈｐ感染率。结果ＣＯ２－２，ｉＮＯＳ在胃癌中的表达率明显高于浅表性胃炎（

Significance of COX-1, COX-2 and iNOS expression in superficial gastritis,gastric mucosal atypical hyper plasia and gastric carcinoma

Objective To investigate the significance of expression of COX 1,COX 2 and iNOS in superficial gastritis, gastric mucosal atypical hyperplasia and gastric carcinoma and to identify the relationship among COX 2, iNOS, gastric carcinoma and Hp infection. Methods Immunohistochemical methods were used with the polyclonal antibody to COX 1, COX 2 and iNOS. The expression of iNOS, COX 1, COX 2 and the status of Hp infection in 92 cases of paraffin embedded gastric tissues were examined. Of the 92 patients, 33 had superficial gastritis, 30 gastric mucosal atypical hyperplasia and 29 gastric cancer. Hp was detected by Toluidine blue stain. Results Expression of COX 2 and iNOS in gastric cancer was significantly higher than that in gastritis (65.5%,62.1%vs 18.2%, 18.2%, P <0.01). Expression of COX 2 and iNOS in gastritis with Hp infection was higher than that in gastric mucosal atypical hyperplasia with Hp infection. The expression of COX 2 and iNOS was simultaneous in gastritis, gastric mucosal atypical hyperplasia and gastric cancer. Conclusion Inflammation and Hp infection could stimulate the expression of COX 2 and iNOS, which might promote gastric carcinogenesis.