慢性肝病肝组织中TNF-α、TNFR及Bcl-2家族的表达

目的：探讨乙型肝炎病毒相关性慢性肝病肝损伤时TNF－α、TNFR及Bcl-2家族对肝细胞凋亡的调节作用。方法：采用HE染色、免疫组织化学方法检测28例慢性乙型肝炎、22例乙型肝炎后肝硬化患者肝组织中Fas、FasL、Bax、Bcl-2、Bcl-α及Bcl-XL的表达，用分子杂交法检测肝组织中TNF－αmRNA、TNF受体（TNFR) 表达的阳性率及表达程度。结果：各种凋亡蛋白在慢性肝炎组和肝硬化组中的表达无显著差别，但促凋亡因子与抑凋亡因子相比，有显著或极显著差异（P＜0．05，P＜0．01）。TNF－α、TNFR阳性表达率在肝硬化组为90．9％、95．5％，明显高于慢性肝炎组（P＜0．05，P＜0．01）。结论：抑制促凋亡因子、阻止肝细胞大量凋亡对防治慢性肝炎重症化有重要的临床意义。

Expressions of TNF-α、TNFR and Bcl-2 family in liver tissue of chronic liver disease

Objective To study the expressions of tumor necrosis factor-?( TNF-?) .its receptor( TNFR) and Bcl-2 family in liver tissues of chronic hepatitis B,liver cirrhosis in order to elucidate their roles in regulation of hepatocyte apoplosis in these diseases. Methods Liver tissues from 28 cases of chronic hepatitis and 22 cases of liver cirrhosis were obtained through biopsy or surgical operation. HE staining and immunohislochemistry were employed to detect expressions of Fas, FasL, Bax, Bcl-2, Bcl-2? and Bel-XL. mRNA of TNF-? and TNFR were identified with in situ hybridization. Results Positive expressions of TNF-? and TNFR were not significantly different between chronic liver cirrhosis and chronic hepatitis. But There was a significant difference between apoptosis promoters and inhibitors in cirrhosis group and chronic hepatitis group ( P < 0. 05 , P < 0. 01 ). The positive rates of TNF-? and TNFR were 90. 9% and 95. 5% in cirrhosis group respectively, much higher than those in chronic hepatitis group(P < 0. 05 ,P <0. 01 ). Conclusions It was suggested that it should be of clinical significance to inhibit apoplosis promoters so as to prevent extensive apoptosis of liver cells to prevention and treatment of chronic hepatitis.