免疫金银染色在大疱病研究中的意义

三种免疫金银染色法（ＩＧＳＳ）──接法、间接法及葡萄球菌Ａ蛋白金标（ＰＡＧ）法检测自身免疫性大疱病皮损组织３２份，先天性大疱性表皮松解症２例和５例正常人皮肤组织作对照，并与直接免疫荧光（ＤＩＦ）检测结果相比较。同时用包埋后免疫金染色（ＩＧＳ）技术对疱疹样天疱疮和获得性大疱性表皮松解症各１例作免疫电镜研究。结果表明ＩＧＳＳ不仅在光镜水平检测大疱病皮肤组织中自身抗体优于ＤＩＦ，而且可从超微结构上了解免疫复合物沉积的确切部位，对自身免疫性皮肤病的诊断和鉴别诊断具有一定价值。

The Value of IGSS in Studying Bullous Skin Diseases

We used three kinds of immunogold silver staining (IGSS) direct,indirect and Staphylococcus protein A gold (PAG) to investigate the skin lesions 32 cases with various autoimmune bullous diseases, 2 cages with epidermolysis bullous simplex and 5 normal adults as control, and compared with those by DIF. Postembedding immunogold labelling direct IEM was performed on 1 case with pemphigus herpetiformis and 1 case with epidermolysis bullous acquisita. The results demonstrated that IGSS is not only superior to DIF in investigating the autoantibody of the skin lesions by light microscopy but it is able to be used in IEM in autoimmune bullous diseases. We suggest that IGSS have great value in diagnosis and differential diagnosis of autoimmune bullous diseases.