实验性急性化脓性中耳炎中耳粘膜热休克蛋白的表达

目的 :探讨急性化脓性中耳炎时中耳粘膜发生的热休克反应。方法 :向豚鼠中耳注射肺炎克雷伯杆菌 ,制成中耳急性感染动物模型 ,分别于接种后第 1、3、5、7天处死动物取材。应用免疫转印技术动态分析中耳粘膜热休克蛋白 ( HSP)的表达。结果 :正常非应激状态下 ,豚鼠中耳粘膜仅有很弱的 HSP70的表达 ;中耳炎急性期 ,中耳粘膜 HSP70的表达明显升高 ,以第 3天为最强 ,第 3天开始表达 31k D分子 ,出现两条带 ;第 5天最强 ,至第 7天消失。 17k D处第 1～ 5天均有两条带 ,第 3天最高 ,第 7天减弱。结论 :观察发现 ,肺炎克雷伯杆菌感染豚鼠中耳后 ,中耳粘膜出现热休克反应 ,表达 HSP70及与之有相关表位的 HSP31k D和 HSP17k D分子

HSP70 expression of middle ear mucosa in acute suppurativeotitis media

OBJECTIVE: To study HSP70 expression of middle ear mucosa in acute suppurative otitis media. METHOD: An animal model of acute middle ear infection was established by inoculating Klebsiella Pneumoniae into middle ear of guinea pigs. Animals were sacrificed 1, 3, 5 and 7 days after inoculation. The expression of Hsp70 epitope related proteins in middle ear mucosa were analysed by modified western blot test. RESULT: Very light 70 kD bands were recognized by anti Hsp70 monoclonal antibody in the unstressed group. However, middle ear mucosa of the stressed group not only showed relatively strong 70 kD bands, but also expressed more intensely 31 kD and 17 kD bands. The infected mucosa expressed one 70 kD band at 1st day, the strongest band at 3rd day, falling at 5th day; 31 kD was recognized two bands at 3rd day, the strongest bands at 5th day, falling at 7th day; two bands of 17 kD were detected in all days, with the strongest bands at 7th day. CONCLUSION: The results suggested that middle ear mucosa expressed Hsp70 and Hsp70 epitope related proteins like 31 kD and 17 kD in acute otitis media.