| 改良PAGE快速诊断婴幼儿轮状病毒肠炎 |
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| 引用本文： | 胡姣荣,杨菊生,殷邦佑,张淑仙.改良PAGE快速诊断婴幼儿轮状病毒肠炎[J].武汉大学学报(医学版),1989(3). |
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| 作者姓名： | 胡姣荣 杨菊生 殷邦佑 张淑仙 |
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| 作者单位： | 湖北医学院儿科研究所，湖北医学院儿科研究所，湖北医学院附一院儿科，湖北医学院附一院儿科 |
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| 摘 要： | 我们对常规的PAGE法进行改进,不用浓缩胶,缩短了电泳、染色时间;应用本改良PAGE法与ELISA法进行比较,检测39例2岁以下患儿腹泻大便,其结果用改良PAGE法检测为23例阳性、16例阴性,用ELISA法检测为18例阳性、21例阴性。改良PAGF法能直接检测出轮状病毒的核酸区带,既比ELISA法敏感,又比常规的PAGE法快速。
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| 关 键 词： | 轮状病毒 电泳 聚丙酰凝胶/诊断应用 革新推广 |
Rapid Diagnosis of Infantile Rotavirus Enteritis with Modified PAGE |
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| Hu Jiaorong,.Rapid Diagnosis of Infantile Rotavirus Enteritis with Modified PAGE[J].Medical Journal of Wuhan University,1989(3). |
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| Authors: | Hu Jiaorong |
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| Abstract: | We improved the routine PAGE for shortening the time of electrophoresis and stain.The results of modified PAGE and ELISA were compared.Fecal specimens of 39 under-two-year old infants with diarrhea were examined;23 samples were positive and 16 samples negative with modified PAGE;while 18 samples positive and 21 samples negative with ELISA.It proves that modifi- ed PAGE was more sensitive than ELISA,more rapid and simpler than routine PAGE as well.This method is one of the recommended ways in cli- nic dlgnosis of infantile. |
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| Keywords: | rotavirus electrophoresis polycrylamide GEL/DU diffusion of innovation |
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