| Follicle-stimulating Hormone(FSH) Induced Internalization of Porcine FSH Receptor in Cultured Porcine Granulosa Cells and Chinese Hamster Ovary Cells Transfected with Recombinant Porcine FSH Receptor cDNA |
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| 作者姓名： | 朱长虹 田红 熊忠明 肖惠珠 |
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| 作者单位： | ZHU Changhong，TIAN Hong，XIONG Zhongming，XIA Huizhu Family Planning Research Institute，Center for Reproductive Medicine，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030 |
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| 基金项目： | This project was supported by a grant from Hubei Provincial Natural Sciences Foundation (No. 99J15 8) and HubeiScience and Technology Departm ent Foundation |
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| 摘 要： | It is now well known thatthe binding of severalprotein ligands to specific cell- surface receptors is fol-lowed by the internalization and( or) degradation ofthe bound protein.The process of internalizationcould be observed by using the methods of fluores-cent,ferritin- labeled,gold- labeled,and 12 5I- labeledligands1] .The results from such experiments havedelineated a pathway( called receptor- mediated endo-cytosis) thatinvolves the internalization of the boundligand via coated pits and v…
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| 收稿时间： | 26 December 2000 |
Follicle-stimulating hormone (FSH) induced internalization of porcine FSH receptor in cultured porcine granulosa cells and chinese hamster ovary cells transfected with recombinant porcine FSH receptor cDNA |
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| Zhu Changhong,Tian Hong,Xiong Zhongming,Xia Huizhu.Follicle-stimulating Hormone(FSH) Induced Internalization of Porcine FSH Receptor in Cultured Porcine Granulosa Cells and Chinese Hamster Ovary Cells Transfected with Recombinant Porcine FSH Receptor cDNA[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2001,21(3):188-190. |
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| Authors: | Zhu Changhong Tian Hong Xiong Zhongming Xia Huizhu |
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| Institution: | (1) Family Planning Research Institute, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan |
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| Abstract: | In order to study the fate of human follicle-stimulating hormone (FSH) when hormone binds to its receptor, a quick biochemical method that can differentiate between the surface-bound and internalized hormone was used to determine the internalization induced by FSH in cultured both porcine granulosa cells and Chinese hamster ovary (CHO) cells expressing recombinant porcine FSH receptor. The results showed that FSH was slowly internalized, and the internalized radioactivity (acid resistant) reached a peak 10-12 h after addition of 125 I-hFSH. It was suggested that FSHR do not get internalized rapidly under physiological circumstances precisely because the appropriate sequences are absent. |
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| Keywords: | follicle-stimulating hormone internalization granulosa cells |
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