| 抗生素S_(632)A_3在体外抗肠道病毒及单纯疱疹病毒I型(HSV-1)作用的初步研究 |
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| 引用本文： | 何健民,李晓眠,刘民,李电东,刘琼岩.抗生素S_(632)A_3在体外抗肠道病毒及单纯疱疹病毒I型(HSV-1)作用的初步研究[J].天津医科大学学报,2001,7(2). |
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| 作者姓名： | 何健民 李晓眠 刘民 李电东 刘琼岩 |
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| 作者单位： | 1. 天津医科大学微生物学教研室,
2. 中国协和医科大学中国医学科学院医药生物技术研究所 |
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| 基金项目： | 国家自然科学基金课题部分项目 (编号 :39670019) |
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| 摘 要： | 目的 :体外测定抗生素S632A3 抗肠道病毒及单纯疱疹病毒I型的作用 ,为筛选新型抗病毒药物提供参考依据。方法 :用不同稀释度的抗生素S632A3 抑制3种肠道病毒及单纯疱疹病毒I型对Vero细胞的感染作用 ,48h后观察细胞病变效应 ,并用MTT法测定细胞活性。结果 :抗生素S632A3 能明显抑制肠道病毒及疱疹病毒对Vero细胞的致病变作用 ,使细胞存活率升高。结论 :该药物有较显著的抗肠道病毒及单纯疱疹病毒I型的作用 ,且对细胞的毒性很低 ,是一种具有潜在开发前景的药物。
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| 关 键 词： | 抗生素S632A3 肠道病毒 单纯疱疹病毒I型 Vero细胞 |
Inhibitory effects of antibiotic S_(632)A_3 on enterovirus and HSV-1 in vitro |
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| HE Jian-min,LI Xiao-mian,LIU Min,LI Dian-dong,LIU Qiong-yan.Inhibitory effects of antibiotic S_(632)A_3 on enterovirus and HSV-1 in vitro[J].Journal of Tianjin Medical University,2001,7(2). |
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| Authors: | HE Jian-min LI Xiao-mian LIU Min LI Dian-dong LIU Qiong-yan |
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| Institution: | HE Jian-min1,LI Xiao-mian1,LIU Min1,LI Dian-dong2,LIU Qiong-yan2 |
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| Abstract: | Objective: To assay the inhibitory effects of antibiotic S 632A 3 on enterovirus and herpes simplex type-1 virus(HSV-1) in vitro in order to select a new kind of antiviral drug. Methods: Vero cells were infected by different enteroviruses and were cultured with serial dilutions of S 632A 3.The inhibitory effects of S 632A 3 were evaluated with cell survival rates by MTT method and CPE.Results: The cytopathogenic effects (CPE)of cells infected by enterovirus or HSV-1 were inhibited significantly, resulting in higher cell survival rates. Conclusion:The results have shown that S 632A 3 had obviously enterovirus-inhibitory efficacy and low cytotoxity,therefore,it may become a potential candidate in drug development and deserves further research on animal experiments and antiviral mechanisms. |
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| Keywords: | Antibiotic S 632A 3 Enterovirus HSV-1 Verocell |
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