Human vascular endothelial growth factor cDNA cloning and expression in osteoblasts |
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| Authors: | Yang Cao Yang Shuhua Qu Shen |
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| Institution: | 1. Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong Universityof Science and Technology,
2. Department of Biochemistry, Tongji Medical College, Huazhong Universityof Science and Technology, |
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| Abstract: | Human vascular endothelial growth factor cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD hVEGF 165 recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD hVEGF 165 plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly. |
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| Keywords: | vascular endothelial growth factor gene amplification gene expression |
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