| 菊苣酸诱导人白血病细胞HL-60凋亡作用及机制研究 |
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| 引用本文： | 陈瑞奇,;罗桂芳,;张宗利,;陈文丽,;曾光尧,;信红亚.菊苣酸诱导人白血病细胞HL-60凋亡作用及机制研究[J].中南药学,2014(12):1208-1210. |
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| 作者姓名： | 陈瑞奇 ;罗桂芳 ;张宗利 ;陈文丽 ;曾光尧 ;信红亚 |
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| 作者单位： | [1]中南大学湘雅医院血液科,长沙410008; [2]湖南省药物安全评价研究中心,长沙410331; [3]中南大学湘雅医学院临床医学系,长沙410013; [4]中南大学药学院,长沙410013 |
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| 基金项目： | 湖南省科技计划项目（No.2011SK3225） |
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| 摘 要： | 目的观察菊苣酸诱导人急性髓系白血病细胞株HL-60凋亡的作用并初步探讨其机制。方法培养HL-60细胞,分别给予终浓度为10100μmol·L-1的菊苣酸48 h,检测细胞活性和凋亡、Caspase-3活性以及Bcl-2蛋白表达水平。结果终浓度为10100μmol·L-1的菊苣酸48 h,检测细胞活性和凋亡、Caspase-3活性以及Bcl-2蛋白表达水平。结果终浓度为10100μmol·L-1的菊苣酸能呈浓度依赖性降低HL-60细胞增殖活性和增加凋亡,增强Caspase-3活性和下调Bcl-2蛋白表达。结论菊苣酸具有抑制白血病细胞株HL-60增殖活性和诱导其凋亡作用,其机制与降低抗凋亡蛋白Bcl-2表达及增强Caspase-3活性有关。
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| 关 键 词： | 菊苣酸 白血病细胞 凋亡 |
Apoptotic effect of chicoric acid on human leukemia cell line HL-60 |
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| Institution: | CHEN Rui-qi, LUO Gui-fang, ZHANG Zong-li, CHEN Wen-li, ZENG Guang-yao, XIN Hong-ya (1. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008; 2. Hunan Research Center for Safety Evaluation of Drugs, Changsha 410331; 3. Department of Clinical Medicine, Xiangya School of Medicine, Central South University, Changsha 410013; 4. School of Pharmaceltical Sciences, Central South University, Changsha 410013) |
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| Abstract: | Objective To observe the apoptotic effect of chicoric acid on human leukemia cell line HL-60. MethodsHL-60 cells were cultured and incubated wi th chicoric acid（final concentration： 10- 100 μmol·L^- 1） for 48 h. The cell viability and apoptosis, Caspase-3 activity and expression of Bcl-2 protein were determined. Results Chicoric acid（final concentration： 10- 100 μmol·L^- 1） concentration-dependently decreased the cell proliferative viability and increased the apoptosis in HL-60 cells. The enhance of Caspase-3 activity and down-regulation of Bcl-2 protein expression were observed in HL-60 cells treated with chicoric acid. Conclusion Chicoric acid can inhibit the viability and induce apoptosis in HL-60 cells, which may be related to the expression down-regulation of anti-apoptotic protein Bcl-2 and increase of Caspase-3 activity. |
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| Keywords: | chicoric acid leukemia cell apoptosis |
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