首页 | 本学科首页   官方微博 | 高级检索  
     

菊苣酸诱导人白血病细胞HL-60凋亡作用及机制研究
引用本文:陈瑞奇,;罗桂芳,;张宗利,;陈文丽,;曾光尧,;信红亚. 菊苣酸诱导人白血病细胞HL-60凋亡作用及机制研究[J]. 中南药学, 2014, 0(12): 1208-1210
作者姓名:陈瑞奇,  罗桂芳,  张宗利,  陈文丽,  曾光尧,  信红亚
作者单位:[1]中南大学湘雅医院血液科,长沙410008; [2]湖南省药物安全评价研究中心,长沙410331; [3]中南大学湘雅医学院临床医学系,长沙410013; [4]中南大学药学院,长沙410013
基金项目:湖南省科技计划项目(No.2011SK3225)
摘    要:目的观察菊苣酸诱导人急性髓系白血病细胞株HL-60凋亡的作用并初步探讨其机制。方法培养HL-60细胞,分别给予终浓度为10100μmol·L-1的菊苣酸48 h,检测细胞活性和凋亡、Caspase-3活性以及Bcl-2蛋白表达水平。结果终浓度为10100μmol·L-1的菊苣酸48 h,检测细胞活性和凋亡、Caspase-3活性以及Bcl-2蛋白表达水平。结果终浓度为10100μmol·L-1的菊苣酸能呈浓度依赖性降低HL-60细胞增殖活性和增加凋亡,增强Caspase-3活性和下调Bcl-2蛋白表达。结论菊苣酸具有抑制白血病细胞株HL-60增殖活性和诱导其凋亡作用,其机制与降低抗凋亡蛋白Bcl-2表达及增强Caspase-3活性有关。

关 键 词:菊苣酸  白血病细胞  凋亡

Apoptotic effect of chicoric acid on human leukemia cell line HL-60
Affiliation:CHEN Rui-qi, LUO Gui-fang, ZHANG Zong-li, CHEN Wen-li, ZENG Guang-yao, XIN Hong-ya (1. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008; 2. Hunan Research Center for Safety Evaluation of Drugs, Changsha 410331; 3. Department of Clinical Medicine, Xiangya School of Medicine, Central South University, Changsha 410013; 4. School of Pharmaceltical Sciences, Central South University, Changsha 410013)
Abstract:Objective To observe the apoptotic effect of chicoric acid on human leukemia cell line HL-60. MethodsHL-60 cells were cultured and incubated wi th chicoric acid(final concentration: 10- 100 μmol·L^- 1) for 48 h. The cell viability and apoptosis, Caspase-3 activity and expression of Bcl-2 protein were determined. Results Chicoric acid(final concentration: 10- 100 μmol·L^- 1) concentration-dependently decreased the cell proliferative viability and increased the apoptosis in HL-60 cells. The enhance of Caspase-3 activity and down-regulation of Bcl-2 protein expression were observed in HL-60 cells treated with chicoric acid. Conclusion Chicoric acid can inhibit the viability and induce apoptosis in HL-60 cells, which may be related to the expression down-regulation of anti-apoptotic protein Bcl-2 and increase of Caspase-3 activity.
Keywords:chicoric acid  leukemia cell  apoptosis
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号