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RP-HPLC测定茯苓乙醇提取物中茯苓酸的含量
引用本文:王光忠,邵贝贝,邓媛媛,黄婕,刘焱文.RP-HPLC测定茯苓乙醇提取物中茯苓酸的含量[J].中药研究与信息,2012(1):41-42,50.
作者姓名:王光忠  邵贝贝  邓媛媛  黄婕  刘焱文
作者单位:湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,湖北武汉430061
基金项目:国家自然科学基金项目(30970295)
摘    要:目的:建立茯苓乙醇提取物中茯苓酸的含量测定方法,为该提取物的质量控制提供依据。方法:采用反相高效液相色谱法(RP—HPLC)对茯苓乙醇提取物中茯苓酸进行定性定量分析,利用保留时间定性和外标两点法定量。结果:此方法线性关系良好,茯苓酸线性回归方程为Y:565.41X+14.477(r=0.9995),平均回收率为99.76%,RSD=1.13%(n=6)。结论:该方法简便、可靠,可作为茯苓乙醇提取物中茯苓酸的含量测定方法。

关 键 词:茯苓乙醇提取物  茯苓酸  RP—HPLC  含量分析

Determination of Pachymic Acid in Ethanol Extract of Poria cocos by RP-HPLC
WANG Guang-zhong,SHAO Bei-bei,DENG Yuan-yuan,HUANG Jie,LIU Yan-wen.Determination of Pachymic Acid in Ethanol Extract of Poria cocos by RP-HPLC[J].Research & Information on Traditional Chinese Medicine,2012(1):41-42,50.
Authors:WANG Guang-zhong  SHAO Bei-bei  DENG Yuan-yuan  HUANG Jie  LIU Yan-wen
Abstract:Objective: To determine the content of pachymie acid in ethanol extract of Poria cocos and provide the basis for the effective quality control of the material. Methods: Paehymic acid in ethanol extract of Poria cocos is derterminated by RP-HPLC with the retention time qualitative and external standard method quantitative. Results: The linear regression equation is Y = 565.41X + 14. 477 (r = 0. 999 5 ) , the average recovery is 99.76 % , RSD = 1.13 % (n = 6). Conclusion: The method is simple and reliable and can be used as a a quality control for ethanol extract of Poria cocos.
Keywords:Ethanol extract of Poria cocos  Pachymic acid  RP-HPLC  Content analysis
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