不同来源骨髓间充质干细胞对CD34+细胞增殖的影响**

背景：作者前期研究显示,银屑病患者与正常人骨髓间充质干细胞的生物学特性、免疫学反应及抗原提呈功能有明显差异,而与流产胎儿骨髓间充质干细胞无明显差异。目的：观察不同来源骨髓间充质干细胞对正常人骨髓CD34+细胞增殖的影响。方法：密度梯度离心法分离银屑病患者和流产胎儿骨髓单一核细胞并培养、传代,传至第2代72 h后收集培养上清液,流式细胞仪鉴定骨髓CD34+细胞及骨髓间充质干细胞纯度。倒置显微镜下观察培养细胞的生长状态,细胞磁珠分选仪分选出的正常人骨髓CD34+细胞加入骨髓间充质干细胞培养上清液培养24 h 后,四甲基偶氮唑盐比色法检测骨髓CD34+细胞的增殖活性。结果与结论：CD34+细胞加入银屑病患者和流产胎儿骨髓间充质干细胞培养上清培养24 h后,细胞形态无差别。银屑病患者和流产胎儿骨髓间充质干细胞培养上清液对正常人骨髓CD34+细胞增殖的影响差异无显著性意义(P > 0.05)。

Effect of different sources of bone mesenchymal stem cells on CD34+ cell proliferation

BACKGROUND: There are some significant differences of biological characteristics, immunological reaction and antigen presentation between bone mesenchymal stem cells (BMSCs) from psoriasis vulgaris (PV) patients and normal health. But there are no more differences between PV patients and aborted fetus. OBJECTIVE: To study the effect of different sources of BMSCs on CD34+ cell proliferation. METHODS: Mononuclear cells were separated from bone marrow in PV patients and aborted fetus by density gradient centrifugation method, then the cells were cultivated and generated. The cultured supernatant was collected until 72 hours later by spreading to the second generation. The purity of CD34+ cells and BMSCs was detected by flow cytometry. Cells growth was observed under inverted microscope. CD34+ cells from normal human bone marrow were selected by magnetic cell sorting method, and then the cells were cultured in the BMSCs culture supernatant for 24 hours. MTT was used to detect the proliferative activity of CD34+ cells.RESULTS AND CONCLUSION: There was no statistic difference on the morphology of CD34+ cells after cultured in the BMSCs culture supernatant derived from PV patients and aborted fetus for 24 hours. There is no significantly effect of BMSCs culture supernatant derived from PV patients and aborted fetus on the proliferation of CD34+ cells (P > 0.05).