改良Alamar Blue法检测结直肠微小组织块的活力***☆

背景：Alamar Blue法是测定组织细胞活性的方法之一，目前多数应用在细胞系活力测定方面，尚未见应用于微小组织块活力的评价。目的：采用改良Alamar Blue法评价结直肠正常黏膜组织块和癌组织块的活力。方法：以细胞活力测定的Alamar Blue法为基础，应用单位质量吸光度来评价结直肠正常黏膜组织块和癌组织块的活力。根据检测试剂盒说明书和预实验结果，实验选择0，6，12，18，24，30，36 h作为检测时间点，每一例标本分为7组，设空白对照孔。结果与结论：单位质量吸光度能够随培养时间的推移，很好地反映组织块增殖活力的变化趋势，结直肠正常黏膜组织块和癌组织块培养12~24 h达到良好的增殖状态，证实改良Alamar Blue法是评价结直肠微小组织块灵敏有效的新型检测方法之一。

Viability of tissue fragments in colon and rectum detected using modified Alamar Blue assay

BACKGROUND: Alamar Blue assay is one of the common methods to evaluate histic or cellular viability. It is widely used to detect cell viability at present. However, using Alamar Blue assay to detect the viability of tissue fragments has not been reported. OBJECTIVE: To detect the viability of the regular tissue fragments and cancer tissue fragments in the mucous membrane of colon and rectum using modified Alamar Blue assay.METHODS: Based on cell viability detect assay of Alamar Blue, the viability of the regular tissue fragments and cancer tissue fragments in the mucous membrane of colon and rectum was assessed using absorbance per unit mass. The detecting time was chosen at 0, 6, 12, 18, 24 , 30 and 36 hours according to the specification of the Alamar Blue assay and the pre-experiment results. Every specimen was divided into seven groups and the blank control hole was established.RESULTS AND CONCLUSIONS: Absorbance per unit mass reflected the change tendency of proliferation activity in tissue fragments as the culture time went on. Both regular tissue fragments and cancer tissue fragments in the mucous membrane of colon and rectum reached the best proliferative state at 12-24 hours after culture. It confirms that the modified Alamar Blue assay is a sensitive and effective way to evaluate the viability of colorectal tissue fragments.