沙鼠肝泡球蚴组织中骨桥蛋白与IL-10的表达及相关性研究

目的 检测沙鼠肝泡状棘球蚴组织中骨桥蛋白（OPN）及白细胞介素-10（IL-10）的表达，并进一步探讨两者的相关性。方法 20%泡状棘球蚴组织混悬液开腹直视下肝脏穿刺注射感染长爪沙鼠60只，每只0.1mL，建立肝泡状棘球蚴长爪沙鼠模型。随机分为模型组（无干预，40只）和实验组（抗OPN抗体干预，20只）。感染至100 d时剖杀所有长爪沙鼠，观察泡状棘球蚴生长和转移情况，取长爪沙鼠肝泡状棘球蚴组织和转移灶标本。采用免疫组织化学SP法观察沙鼠肝泡状棘球蚴组织和转移灶中OPN及IL-10的表达情况。 结果 60只长爪沙鼠全部感染肝泡状棘球蚴。免疫组化染色显示肝泡状棘球蚴组织可见OPN及IL-10不同程度的表达。模型组中OPN和IL-10阳性细胞表达率分别为72.5%（29/40）和65%（26/40），OPN及IL-10主要分布在肝泡状棘球蚴纤维囊壁，阳性产物均定位于细胞浆。65%（26/40）的长爪沙鼠模型经病理证实发生胸廓内淋巴结转移，伴有胸廓淋巴结转移的肝泡状棘球蚴组织中OPN及IL-10阳性细胞表达率分别为84.6% (22/26)、76.9%(20/26)，均高于未发生胸廓淋巴结转移的肝泡状棘球蚴组织（50%、42.9%）（P﹤0.05）。实验组OPN和IL-10的阳性细胞表达率分别为40%（8/20）和35%(7/20)。OPN与IL-10表达呈正相关（r=0.605，P=0.000）。结论 OPN阳性表达可能与肝泡状棘球蚴的转移有关，OPN与IL-10可能在肝泡状棘球蚴的生长中起协同作用。

Expressions of osteopontin and interleukin-10 in Gerbil hepatic alveolar hydatid tissue and their correlation

ABSTRACTTo investigate the expressions of osteopontin and interleukin-10 and their correlation in Gerbil hepatic alveolar hydatid tissue, 60 gerbils were infected with 20% E.multilocularis suspension (0.1 mL per gerbil) through abdominal opening injection in liver. They were randomly divided into model group (No intervention, 40 gerbils) and experimental group (Anti-OPN antibody interference, 20 gerbils). Gerbils were sacrificed at 100 days post-infection for observing the growth and metastasis of hepatic Echinococcus cyst. The expressions of OPN and IL-10 in hepatic Echinococcus cyst and metastasis tissue were measured by immunohistochemistry staining (SP method). Results showed that all gerbils infected with hepatic alveolar hydatid. Expression of OPN and IL-10 were found at different degree in Echinococcus cysts. In model group, the positive expression rate of OPN and IL-10 in Echinococcus cysts were 72.5% (29/40) and 65% (26/40). Both OPN and IL-10 were mainly distributed in the germinal layer. The 65% (26/40) of them occurred thoracic lymph node metastases. The OPN and IL-10 expression rate in hepatic Echinococcus cysts with thoracic lymph node metastases were 84.6% (22/26) and 76.9% (20/26), which were significantly higher than that of hepatic Echinococcus cysts without thoracic lymph node metastases (50%, 42.9%) (P<0.05). In experimental group, the positive expression rates of OPN and IL-10 in Echinococcus cysts were 40% (8/20) and 35% (7/20). The expression of OPN was significantly correlated with the expression of IL-10 (r=0.605, P=0.000). In conclusion, OPN might significantly relate to the metastasis of hepatic Echinococcus cysts. OPN and IL-10 might play synergetic roles in hepatic alveolar hydatid tissue.