近红外照射下金纳米链及Anti-EGFR/Au共轭物对人喉癌Hep-2细胞的抑制*☆

背景：金纳米颗粒在近红外激光照射下对肿瘤细胞具有杀伤效应。目的：评价金纳米链及anti-EGFR/Au共轭物近红外照射对人喉癌Hep-2细胞的生长抑制和热杀伤作用。方法：取对数生长期的Hep-2细胞，随机分为空白对照组、单纯照射组、金纳米链照射组和anti-EGFR/Au照射组，加入不同培养液后，各照射组进行近红外激光照射。观察照射后细胞凋亡情况和凋亡程度。结果与结论：倒置显微镜下观察显示空白对照组和单纯照射组Hep-2细胞仍具有活性，金纳米链照射组有明显的损伤，但anti-EGFR/Au照射组损伤程度更重。流式细胞分析显示金纳米链照射组及anti-EGFR/Au靶向照射组均有不同程度的Hep-2细胞凋亡，且照射时间越长，凋亡细胞越多。说明金纳米链近红外照射可以杀伤人喉鳞癌Hep-2细胞，anti-EGFR/Au靶向照射比金纳米链照射效果更好，并呈现一定照射时间的依赖性。关键词：金纳米链；Hep-2细胞；流式细胞分析；照射；生物材料与纳米技术doi:10.3969/j.issn.1673-8225.2012.12.013

Apoptosis of human laryngeal cancer cells induced by gold nanochain and anti-epidermal growth factor receptor/Au conjugate under near-infrared hyperthermia

BACKGROUND: The gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.OBJECTIVE: To evaluate the growth inhibition and heat killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugate on human laryngeal cancer Hep-2 cells under near-infrared hyperthermia.METHODS: The Hep-2 cells in the logarithmic growth phase were collected and divided into blank control group, irradiation group, gold nanochain irradiation group and anti-EGFR/Au irradiation group. After added with different culture medium, the near-infrared laser irradiation was performed in each group. Then the apoptosis of Hep-2 cells in different experimental groups was observed after the near-infrared laser irradiation. RESULTS AND CONCLUSION: Inverted microscope observation showed that the Hep-2 cells in the blank control and irradiation groups were still active and the Hep-2 cells in the gold nanochain irradiation group were damaged significantly, while the Hep-2 cells in anti-EGFR/Au irradiation group were damaged more serious. Flow cytometry analysis showed that the Hep-2 cells in the gold nanochain irradiation and anti-EGFR/Au irradiation groups had a varying degree of apoptosis and the increasingly heavy damage with the time. The gold nanochain irradiation can kill Hep-2 cells and the anti-EGFR/Au irradiation has a better effect in a time-depended manner.