| 乙醇改变骨髓间充质干细胞神经肽的表达* |
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| 引用本文： | 王义生,朱绍阳,李月白,赵国强,张战峰. 乙醇改变骨髓间充质干细胞神经肽的表达*[J]. 中国组织工程研究, 2012, 16(6): 981-984. DOI: 10.3969/j.issn.1673-8225.2012.06.007 |
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| 作者姓名： | 王义生 朱绍阳 李月白 赵国强 张战峰 |
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| 作者单位： | 1郑州大学第一附属医院骨科,河南省高等学校临床医学重点学科开放实验室,河南省郑州市 450052;郑州大学基础医学院,2生物化学教研室,3微生物免疫教研室,河南省郑州市 450001 |
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| 基金项目： | 国家自然科学基金资助项目(81171776),课题名称：hCGRPα基因修饰BMSCs联合自体骨移植治疗股骨头坏死的实验研究。 |
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| 摘 要： | 背景：研究发现骨髓间充质干细胞的分化受神经系统调控。目的：观察乙醇对人骨髓间充质干细胞降钙素基因相关肽受体、P物质受体、过氧化物酶体增殖子活化受体γ mRNA及Runx2 mRNA表达的影响。方法：将第2代人骨髓间充质干细胞随机分组培养：实验组加入0.09 mol/L乙醇,对照组正常培养。结果与结论：培养11 d后,实验组降钙素基因相关肽受体、P物质受体、Runx2 mRNA表达较对照组明显降低(P < 0.01),过氧化物酶体增殖子活化受体γ mRNA水平较对照组明显增高(P < 0.01)。表明乙醇改变了骨髓间充质干细胞中神经肽类物质的表达,减弱了其向成骨细胞方向分化。
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| 关 键 词： | 乙醇 神经肽 人源性骨髓间充质干细胞 降钙素基因相关肽 P物质 |
| 收稿时间： | 2011-11-28 |
Effects of alcohol on the expression of neuropeptide in human bone marrow mesenchymal stem cells |
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| Wang Yi-sheng,Zhu Shao-yang,Li Yue-bai,Zhao Guo-qiang,Zhang Zhan-feng. Effects of alcohol on the expression of neuropeptide in human bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(6): 981-984. DOI: 10.3969/j.issn.1673-8225.2012.06.007 |
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| Authors: | Wang Yi-sheng Zhu Shao-yang Li Yue-bai Zhao Guo-qiang Zhang Zhan-feng |
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| Affiliation: | 1Departmemt of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Subjects of Clinical Medicine of Key Subjects, Zhengzhou 450052, Henan Province, China; 2Department of Biochemistry; 3Department of Microbiology & Immunology, Basic Medical College of Zhengzhou University, Zhengzhou 450001, Henan Province, China |
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| Abstract: | BACKGROUND: Studies have found that the nervous system can effect the differentiation of bone marrow mesenchymal stem cells (BMSCs). OBJECTIVE: To investigate the effect of alcohol on the expressions of calcitontin gene-related peptide receptor (CGRPR) mRNA, substance P receptor (SPR) mRNA, peroxisom proliferator-activeted receptor-γ (PPARγ) mRNA and Runx2 mRNA in human BMSCs. METHODS: The second generation of BMSCs were divided into two groups at random. The cells in experimental group were treated with 0. 09 mol/L alcohol, and the control group was not treated. RESULTS AND CONCLUSION: The expressions of FGF mRNA, CGRPR mRNA, SPR mRNA and Runx2 mRNA in the experimental group were significantly lower those that in the control group (P < 0.01), but the expression of PPARγ mRNA was increased compared with control group (P < 0.01). Alcohol could change the expressions of neuropeptide and depress the differentiation to osteoblasts. |
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