消退素RvE1对日本血吸虫病肝纤维化小鼠外周血肝纤维化指标和细胞因子的影响

目的：通过检测模型小鼠外周血肝纤维化有关指标和细胞因子水平研究消退素RvE1对小鼠日本血吸虫病肝纤维化的影响及其机制，从而探讨合理的防治策略。方法：用日本血吸虫尾蚴皮肤敷贴法感染小鼠，构建日本血吸虫病肝纤维化模型。感染5wk后，将造模组小鼠随机分为2组：模型对照组(血吸虫感染+NS.)、治疗组（血吸虫感染+RvE1），以正常小鼠为正常对照组。感染10wk后取血标本，采用放射免疫法检测血清肝纤维化指标，包括Ⅳ型胶原（Ⅳ-C）、Ⅲ型前胶原（PCⅢ）、透明质酸（HA）及层粘连蛋白（LN）；采用ELISA 法测定RvE1对日本血吸虫病肝纤维化小鼠血清中TNF-α和IFN-γ水平的影响。结果：感染10wk后，各组小鼠未见自然死亡，各组小鼠体重无明显变化，统计学分析无差异；感染10wk后，RvE1治疗组小鼠血清肝纤维化各项指标，包括Ⅳ型胶原、Ⅲ型前胶原、透明质酸及层粘连蛋白水平，与模型对照组比较均有明显降低，有显著性差异（P<0.05）；感染10wk后，RvE1治疗组小鼠血清中促炎介质TNF-α水平较模型对照组有明显降低，有显著性差异（P<0.05），而血清中抑炎介质IFN-γ水平较模型对照组有明显升高，有显著性差异（P<0.05）。结论：我们认为给予外源性RvE1可以通过抗炎及促进肝脏炎症消退，发挥抗日本血吸虫病肝纤维化的作用。

Effect of Resolvin E1 on serum of cytokines and liver fibrosis index in mice with schistosomiasis japonica

AIMTo investigate the effect and mechanism of Resolvin E1 on liver fibrosis mice models with schistosomiasis japonica by detecting the changes of serum levels of cytokines and liver-fibrosis. METHODSMice were infected with schistosoma japonicum cercariae percutaneously, to construct mice models of liver fibrosis of schistosomiasis japonica. The infected mice were divided randomly into three groups at the end of the 5th week after infectionmodel group, model control group (to inject normal saline), treated group (to inject RvE1) and normal control group. The mice were killed at the end of the 10th week. The serum level of collagen IV (C-IV)，precollagen Ⅲ (PCⅢ), hyaluronic acid (HA) and laminin (LN) were evaluated by radioimmunoassay (RIA). The serum level of TNF-α and IFN-γ were evaluated by ELISA. RESULTSAfter infection 10wk, no mice naturally died, and the weight of mice was no significant changes in each group. The serum level of liver fibrosis index in RvE1 treated mice,including the collagen IV (C-IV)，precollagen Ⅲ (PCⅢ), hyaluronic acid (HA) and laminin (LN), were significantly lower than with the model control group, and there was a significant difference (P <0.05). The serum level of TNF-α in RvE1 treated mice, was significantly lower than with the model control group, and there was a significant difference (P <0.05). The serum level of IFN-γ in RvE1 treated mice, was significantly high than with the model control group, and there was a significant difference (P <0.05).