尿酸对人胎盘间充质干细胞增殖的影响

背景：尿酸对不同细胞具有不同的作用,那么,尿酸对人胎盘源间充质样干细胞是促进还是抑制呢？目的：观察尿酸对人胎盘间充质干细胞增殖的影响及对胎盘间充质干细胞作用的时间-效应及剂量-效应关系。方法：以体外培养人胎盘间充质干细胞为研究对象,分为6组,不加尿酸的对照组,和加入不同浓度的尿酸0.1,0.2,0.4,0.6,0.8 mmol/L组。结果与结论：同一时间内,随着尿酸浓度增加,细胞数目逐渐增加,尤其是0.8 mmol/L尿酸组;同一浓度尿酸,随着培养时间的延长(时间< 5 d),细胞数目也逐渐增加,第5天的时候细胞数目达到高峰。提示尿酸具有呈时间及浓度依赖性的促进胎盘间充质干细胞增殖的作用,尤其是0.8 mmol/L尿酸在培养第5天的时候促进作用最明显。关键词：尿酸;浓度;人胎盘间充质干细胞;增殖;细胞数目doi:10.3969/j.issn.1673-8225.2012.10.019

Effect of uric acid on proliferation of human placenta-derived mesenchymal stem cells

BACKGROUND: Uric acid (UA) plays different roles on different cells. But, what is the effect of UA on the proliferation of human placenta-derived mesenchymal stem cells (hPMSCs), promoting or suppression? 0BJECTIVES: To observe the effect of UA on proliferation of hPMSCs, and to explore the relationship between time-effect and dose-effect. METHODS: The hPMSCs cultured in vitro were used and divided into 6 groups: the control group and the UA group which contained 0.1, 0.2, 0.4, 0.6, 0.8 mmol/L UA. RESULTS AND CONCLUSION: MTT assay showed that the number of hPMSCs in 0.1, 0.2, 0.4, 0.6, 0.8 mmol/L UA group was more than that in the control group at the same time, especially in 0.8 mmol/L UA group; the number of hPMSCs in same concentration of UA was increasing following the time (time﹤5 d), and reached to peak at the 5th day. UA could accelerate the proliferation of hPMSCs and showed a time- and dose-dependent manner, and the effect of 0.8 mmol/L UA on the proliferation capacity of hPMSCs was stronger than others at the 5th day.