成骨细胞培养在骨组织工程和运动领域的研究进展

背景：成骨细胞在不同的培养环境、细胞因子刺激下增殖、分化及功能不同。目的：总结成骨细胞体外培养技术与成骨细胞增殖速度的研究进展。方法：检索1990/2011PubMed数据及万方数据库有关运动、成骨细胞培养和骨组织工程等方面的文献,英文检索词为“exercise, Osteoblast raise method”,中文检索词为“运动,成骨细胞,骨组织工程”。排除与研究目的无关和内容重复者。保留29篇文献做进一步分析。结果与结论：骨组织工程构建中种子细胞的选择方式、支架材料的不同形式、细胞因子、培养环境、力学因素都对成骨细胞的培养有重要影响。构建骨组织工程中成骨细胞在不同的培养环境、细胞因子刺激下会有不同的增殖能力和分化结果。成骨细胞的增殖速度与旋转壁式生物反应器提供的低剪力环境、适宜的细胞因子、细胞支架的选择密切相关。关键词：成骨细胞;培养;骨组织工程;运动;细胞增殖doi:10.3969/j.issn.1673-8225.2012.11.033

Osteoblasts culture in bone tissue engineering and exercise research

BACKGROUND: Under different culture and cytokine-stimulated conditions, steoblasts have different proliferation and differentiation abilities as well as functions.  OBJECTIVE: To summarize the progress in in vitro culture of osteoblasts as well as proliferative ability of osteoblasts. METHODS: A search of PubMed and Wanfang databases (1990/2011) was performed for articles addressing exercise, osteoblast culture and bone tissue engineering. The keywords were “exercise, osteoblast raise method” in English and “exercise, osteoblast, bone tissue engineering” in Chinese. Unrelated articles or repetitive articles were excluded, and finally 29 articles were included in result analysis.RESULTS AND CONCLUSION: Osteoblast culture is influenced by the following factors: methods to choose seed cells, pattern of scaffold materials, cytokines, culture environment, and mechanical factors in bone tissue engineering construction. Osteoblasts cultured in different conditions and stimulated by different cytokines have different proliferative and differentiated abilities. The proliferative speed of osteoblasts is closely related to a low shear environment produced by rotating-wall vessel bioreactor, appropriate cytokines and selection of cytoskeleton.