丝素蛋白/壳聚糖三维多孔支架的构建及结构表征** |
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作者姓名: | 柳 磊 曾曙光 任 力 魏柳琼 杨 静 曹彦南 石 勇 |
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作者单位: | 1南方医科大学附属口腔医院,广东省口腔医院,广东省广州市 510280;2华南理工大学材料科学与工程学院,广东省广州市 540641 |
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基金项目: | 广东省中医药局科研基金资助项目(20111145);广东省科技计划项目(2011B031300022)。 |
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摘 要: | 背景:丝素蛋白和壳聚糖均无毒性且具有良好的生物相容性,但是单一成分作为生物支架时都不能满足支架材料的需求。
目的:制备各种不同组分的丝素蛋白及壳聚糖复合支架材料,观察其微观结构及相关性能,筛选出适合成骨细胞生长的理想支架材料。
方法:通过CaCl2∶C2H5OH∶H2O=1∶2∶8(摩尔比)溶解体系溶解、过滤、浓缩提纯,制备出2%的丝素蛋白溶液,壳聚糖溶解于乙酸溶液配制成的3%壳聚糖溶液,将两者以不同的比例相混合,经数次冷冻干燥后,得到成品支架材料。采用电镜观察形貌,计算孔隙率并对支架的结构进行红外、X射线衍射、电子能谱分析观察。
结果与结论:将壳聚糖和丝素蛋白共混后,互为改性,制备出了结构较稳定的支架材料。其中40%丝素蛋白-60%壳聚糖组具有适合成骨细胞生长的较佳孔径,可作为细胞支架的首选配比。
关键词:丝素蛋白;壳聚糖;骨组织工程;支架;生物材料
doi:10.3969/j.issn.1673-8225.2012.12.025
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关 键 词: | 丝素蛋白 壳聚糖 骨组织工程 支架 生物材料 |
收稿时间: | 2011-12-16 |
Preparation and characterization of porous silk fibroin/chitosan scaffolds |
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Authors: | Liu Lei Zeng Shu-guang Ren Li Wei Liu-qiong Yang Jing Cao Yan-nan Shi Yong |
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Institution: | 1Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China; 2Department of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, China |
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Abstract: | BACKGROUND: Silk fibroin and chitosan are non-toxic and have a good histocompatibility. However, they could not satisfy the biological scaffold material needs if working separately.
OBJECTIVE: To explore and analyze the material structure and relate performance for characterization on the basis of making a variety of compound scaffolds with different proportion of silk fibroin and chitosan, and to select the ideal scaffolds that suitable for the growth of osteoblasts.
METHODS: 2% silk fibroin solution was made by dissolution from CaCl2 solvent systems at a mole ratio of CaCl2:C2H5OH:H2O= 1:2:8, filtration, concentration and purification. The chitosan was dissolved in acetic acid solution prepared 3% chitosan-acetic acid solution. The silk fibroin and chitosan were blend in a freeze-drying approach in different proportion in order to get different scaffold materials. Then the morphology of the silk fibroin/chitosan scaffolds was observed under a scanning electron microscope; the porosity was calculated and the structure of the scaffolds was evaluated and observed by Fourier transform infrared spectroscopy, X-ray diffraction and electron spectroscopy.
RESULTS AND CONCLUSIONS: More stable scaffolds were created with the method of blending and modifying silk fibroin and chitosan. The 40% silk fibroin-60% chitosan group proves the preferred ratio of cell scaffold material due to its better aperture for the growth of osteoblasts. |
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