青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用***☆◆

背景：青蒿琥酯是青蒿素低毒、高效的衍生物，具有免疫调节功能，但其具体机制仍需研究阐明。 目的：初步探讨青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用。 方法：建立迟发型超敏反应小鼠模型，40只小鼠随机数字表法均分正常对照组、青蒿琥酯干预组、p-38MAPK抑制剂组、基质对照组进行实验观察。T淋巴细胞转化实验检测小鼠T淋巴细胞增殖水平；Western blot方法检测p38丝裂酶原激活蛋白激酶(p38MAPK)蛋白的活性表达。 结果与结论：局部给药后青蒿琥酯明显减轻迟发型超敏反应小鼠耳肿胀、降低脾指数、抑制刀豆蛋白A诱导的T淋巴细胞增殖；同时减弱p38 MAPK的磷酸化活性表达。提示青蒿琥酯可以有效抑制小鼠迟发型超敏反应，其作用途径可能与抑制p38 MAPK信号通路有关。

Immunomodulatory activity of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice

BACKGROUND: Artesunate is a derivant of high efficiency and low toxicity from artemisinin, which has been shown to affect immune response. However, the underlying mechanism remains elusive. OBJECTIVE: To investigate the immunoregulatory mechanism of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice. METHODS: The effects of artesunate on the ear swelling and spleen index were measured in delayed-type hypersensitivity mice. The transformation of spleen lymphocytes was used to detect the proliferation of T cells. Western blot assay was applied to investigate the p38 mitogen-activated protein kinase (MAPK) activation. The proliferation of T lymphocytes was detected by T lymphocyte transformation test. MAPK activity was detected by western blot method. RESULTS AND CONCLUSION: Topical application of artesunate could suppress the increase in ear thickness, spleen index and ConA-induced T lymphocytes proliferation significantly. Furthermore, artesunate was observed to decrease the phosphorylation of p38 MAPK. These demonstrated that artesunate had an anti-delayed-type hypersensitivity effect through modulating p38MAPK pathways, which might be beneficial for immune-related diseases.