血管内皮生长因子与促血管生成素1对大鼠血管内皮细胞的作用

背景：血管内皮生长因子、促血管生成素1是血管形成过程中始动并且使之持续的重要因子，研究其对血管内皮细胞的作用具有重要的意义。目的：观察血管内皮生长因子与促血管生成素1对培养血管内皮细胞迁移与增殖能力的影响，并探讨其在血管生成方面的作用机制。方法：在大鼠脐静脉内皮细胞内单独或联合加入血管内皮生长因子、促血管生成素1后，划痕实验和MTT检测对细胞迁移与增殖的影响，观察内皮细胞形态、活性、迁移能力。结果与结论：划痕实验显示单独血管内皮生长因子作用时，与空白对照组细胞迁移无明显差异，单独促血管生成素1作用时，不仅不能增加细胞的迁移作用，反较空白对照组有所减弱，当血管内皮生长因子与促血管生成素1联合作用时，细胞迁移较空白对照组明显增强；MTT实验结果表明：单纯加入血管内皮生长因子或促血管生成素1，均不能起到有效促进内皮细胞增殖的作用；联合应用血管内皮生长因子及促血管生成素1可有效促进增殖。结果可见当血管内皮生长因子与促血管生成素1联合应用时，才能有效促内皮细胞迁移与增殖，发挥促血管生成作用。

Effect of vascular endothelial growth factor and angiopoietin-1 on rat vascular endothelial cells

BACKGROUND:Vascular endothelial growth factor and angiopoietin-1 play an important role in the initiating and ongoingprocess of angiogenesis. Study on the effects of vascular endothelial growth factor and angiopoietin-1 on vascular endothelialcells is significant.OBJECTIVE:To explore the effect of vascular endothelial growth factor and angiopoietin-1 on the transfer and proliferation abilityin rat vascular endothelial cells and to explore its mechanism on angiogenesis.METHODS:Rat umbilical vein endothelial cells were treated with vascular endothelial growth factor and angiopoietin-1respectively or in combination. Scratch test and MTT assay were used to detect the cell transfer and proliferation ability. Themorphology, activity and transfer ability of endothelial cells were observed.RESULTS AND CONCLUSION:According to scratch test, there was no significant difference in cell transfer ability between thecells treated with vascular endothelial growth factor alone and the control cells. The cell transfer ability after treated withangiopoietin-1 individually did not increase; on the contrary, it was weakened compared with that of the control group. Thetransfer ability in cells treated with vascular endothelial growth factor combined with angiopoietin-1 improved significantlycompared with that of the control group. According to MTT assay, neither vascular endothelial growth factor nor angiopoietin-1was found to improve the proliferation ability in rat umbilical vein endothelial cells. Combination of vascular endothelial growthfactor and angiopoietin-1 significantly improved the proliferation of rat umbilical vein endothelial cells. These findings indicate thatthe transfer ability, proliferation ability and angiogenesis in rat umbilical vein endothelial cells are only improved by thecombination of vascular endothelial growth factor and angiopoietin-1.