石膏外固定修复兔髌骨-腱连接点断裂的组织形态学变化

背景：石膏外固定对髌骨-腱连接点断裂的治疗作用肯定,但相关修复机制一直未有深入研究。 目的：观察石膏外固定对髌骨-腱连接点断裂修复的组织形态学改变。 方法：切断髌尖与髌腱连接处并切除远端部分髌骨建立兔髌骨-腱连接点损伤模型,实验组在造模的基础上给予石膏外固定,模型组不予固定,分别在造模后2,4,8周取材。 结果与结论：苏木精-伊红染色、Safranin’O染色和巨噬细胞免疫组化染色及其积分吸光度检测均显示实验组造模后骨-腱结合部组织修复快,软骨细胞及骨组织的形成、分化明显优于模型组,且炎症反应程度轻、消失快。说明外固定可加快骨-腱组织修复进程,促进其愈合。 关键词：骨-腱连接点;外固定;组织形态学;积分吸光度值 doi:10.3969/j.issn.1673-8225.2012.13.019

Effect of plaster external fixation on the histomorphological changes for bone-tendon junction healing

BACKGROUND: Plaster external fixation has a positive effect on bone-tendon junction healing, but there is no in-depth study on the repair mechanism. OBJECTIVE: To investigate the effect of plaster external fixation on the histomorphological changes for bone-tendon junction healing. METHODS: The bone-tendon junction healing models were established by cutting off the junction between the patellar tip and the patellar tendon as well as the distal part of the patella. The models were divided into experimental group and model group. The models in the experimental group were performed with plaster external fixation and those in the model group were without fixation. Each group got the sample at 2, 4 and 8 weeks after operation. RESULTS AND CONCLUSION: Hematoxylin-eosin staining, Safranin’O staining, macrophages immunohistochemistry staining and integral absorbance showed that the tissue edema and adhesion at the bone-tendon junction in the experimental group were better than those in the model group, cartilage cells and bone tissue formation and differentiation in the experimental group were significantly better those those in the model group, and degree of the inflammation in the experimental group was light and disappeared quickly. External fixation can accelerate the process of bone-tendon repair and promote the healing.