低频振动刺激骨髓基质干细胞修复骨缺损核因子受体激活剂/核因子受体激活剂配体/骨保护素通路变化的体内实验*

背景：关于低频振动对体内骨髓基质干细胞成骨分化的实验缺乏报道。 目的：通过体内实验研究不同频率振动刺激骨髓基质干细胞修复骨缺损过程中核因子受体激活剂/核因子受体激活剂配体/骨保护素调节通路的变化,并初步探讨其机制。 方法：取新西兰兔骨髓基质干细胞和脱钙骨基质制备复合物,80只新西兰兔制作骨缺损模型,骨缺损区植入复合物后随机数字表法均分组对照组、12.5,25,50,100 Hz振动组,振动组于第7天开始接受不同频率振动干预5周,振动结束后分别对骨保护素mRNA、核激活因子受体配体mRNA进行检测。 结果与结论：与对照组比较,各振动组骨髓基质干细胞骨保护素、核激活因子受体配体基因表达明显上调(P < 0.05),以25,50 Hz显著(P < 0.01);但100 Hz振动时表达则下调(P < 0.05)。说明给予一定频率振动刺激骨髓基质干细胞修复骨缺损,可能与其促进骨保护素基因表达上调有关,理想的振动频率为25,50 Hz。

Effects of low frequency vibration on osteogenesis of bone marrow stromal cells and receptor activator of nuclear factor-kappa B/receptor activator of nuclear factor kappa-B ligand/osteoprotegerin pathway

BACKGROUND: There have been few studies regarding low frequency vibration stimulating osteogenic differentiation of bone marrow stromal cells in vivo. OBJECTIVE: To investigate the effects of different frequencies of vibration on receptor activator of nuclear factor-kappa B (RANK)/ receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) pathway and preliminarily investigate the underlying mechanism.  METHODS: Eighty New Zealand rabbits of bone defects were established. After transplantation of complex of New Zealand bone marrow stromal stem cells and decalcified bone matrix into bone defect region, rat models were randomly divided into a control group and four vibration groups according to different vibration frequencies (12.5, 25, 50, 100 Hz). The vibration group received different frequencies of vibration for 5 weeks from day 7. After vibration, OPG mRNA and RANKL mRNA expression was detected. RESULTS AND CONCLUSION: Compared with the control group, OPG and RANKL mRNA expression was significantly up regulated in each vibration group (P < 0.05), in particular in 25 and 50 Hz vibration groups (P < 0.01), but the expression was significantly down regulated in the 100 Hz vibration group (P < 0.05). This suggests that vibration at a certain frequency can stimulate bone marrow stromal cells to repair bone defects, which occurs possibly because vibration can promote the up regulation of OPG mRNA expression, with the ideal vibration frequency of 25 and 50 Hz.