骨髓间充质干细胞移植治疗大鼠扩张型心肌病

1泸州医学院附属医院急诊科,四川省泸州市646000;2哈尔滨医科大学附属第二医院心内科,黑龙江省哈尔滨市  150000背景：扩张型心肌病所致的心肌纤维化是心力衰竭的病理基础,目前药物治疗、介入治疗和外科手术均不能替代坏死心肌和彻底改善心脏功能。目的：观察异体骨髓间充质干细胞移植对大鼠扩张型心肌病心脏功能的作用和心肌纤维化的影响。方法：40只Wistar大鼠随机数字表法分为细胞移植组(n=15)、对照组(n=15)和空白组(n=10),前2组建立大鼠扩张型心肌病模型。造模成功4周后细胞移植组注射骨髓间充质干细胞悬液150 μL(含3×106个细胞),对照组和空白组注射等量培养液。结果与结论：与空白组相比,细胞移植组和对照组移植前左室收缩末期内径增加,射血分数和缩短分数明显下降(P < 0.01);移植后4周,细胞移植组超声心动图检查和移植前相比,左室收缩末期内径下降、射血分数和缩短分数明显升高(P < 0.01)。细胞移植组心脏胶原的表达低于对照组(P < 0.05)。与对照组相比,其他2组基质金属蛋白酶2及基质金属蛋白酶9表达明显下降(P < 0.05)。提示骨髓间充质干细胞移植后可改善心肌纤维化及扩张型心肌病鼠的心脏功能。 

Bone marrow mesenchymal stem cells transplantation for treatment of dilated cardiomyopathy in rats

BACKGROUND: Myocardial fibrosis induced by dilated cardiomyopathy (DCM) is the pathological basis of heart failure. At present, drug treatment, interventionaI therapy and surgical intervention cannot ameliorate necrotic myocardium and completely improve cardiac function．OBJECTIVE: To investigate the effect of allogenic bone marrow mesenchymal stem cells (BMSCs) transplantation on cardiac function and myocardial fibrosis in rats with DCM.METHODS: Forty Wistar rats were randomly divided into three groups: cell transplantation group (n=15), control group (n=15) and blank group (n=10). DCM models were established in cell transplantation group and control group. Four weeks after the models were established successfully, rats in cell transplantation group were injected with allogeneic BMSCs 150 μL (containing 3×106 cells). Rats in control group and blank group were also implanted with the culture medium in the same amount.  RESULTS AND CONCLUSION: Compared with the blank group, echocardiography showed that the left ventricular end-systolic inside diameter was increased before transplantation, while the ejection fraction and fractional shortening was decreased significantly (P < 0.01) in cell transplantation group and control group. Four weeks after transplantation, the echocardiography showed that the left ventricular end-systolic inner diameter was decreased, while the ejection fraction and fractional shortening was increased obviously (P < 0.01) in cell transplantation group compared with before transplantation. Expression of cardiac collagen in cell transplantation group was lower than that in control group (P < 0.05). Compared with the control group, the expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 was significantly increased in the other two groups (P < 0.05). In conclusion, bone marrow mesenchymal stem cells (BMSCs) transplantation can improve cardiac function and ameliorate myocardial fibrosis in rats with DCM.