壳聚糖磷脂酰胆碱抑制轻度认知障碍患者血清白细胞介素1β及肿瘤坏死因子α的表达**

背景：壳聚糖磷脂酰胆碱可通过血-脑脊液屏障改善阿尔茨海默病症状。 目的：观察壳聚糖磷脂酰胆碱对轻度认知障碍患者血清白细胞介素1β、肿瘤坏死因子α及简易精神状态量表MMSE评分的影响。 方法：从潍坊市部分社区及老人院按照轻度认知障碍诊断标准筛选36例轻度认知障碍患者,随机分为实验组(口服壳聚糖磷脂酰胆碱治疗)与空白对照组(不服药),并选择年龄、学历相匹配的健康对照。应用放射免疫分析法检测3组血清中白细胞介素1β和肿瘤坏死因子α水平。 结果与结论：实验组治疗后血清白细胞介素1β、肿瘤坏死因子α水平较治疗前明显降低,并明显低于空白对照组(P < 0.05);MMSE评分较治疗前明显提高(P < 0.05)。说明壳聚糖磷脂酰胆碱能改善轻度认知障碍患者认知功能,降低血清白细胞介素1β及肿瘤坏死因子α水平,减轻炎症反应,延缓病情进展。 关键词：壳聚糖磷脂酰胆碱;轻度认知障碍;白细胞介素1β;肿瘤坏死因子α;生物材料 doi:10.3969/j.issn.1673-8225.2012.08.040

Effects of chitosan-phosphatidylcholine complex on serum interleukin 1 beta and tumor necrosis factor alpha in mild cognitive impairment patients

BACKGROUND: Chitosan phosphatidylcholine can improve the symptoms of Alzheimer's disease through the blood-brain barrier. OBJECTIVE: To explore the influence of chitosan-phosphatidylcholine complex on serum interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) and mini-mental state examination (MMSE) in mild cognitive impairement (MCI) patients. METHODS: According to diagnostic criteria of MCI, 36 patients with MCI selected from a part of homes and communities in Weifang City were randomly divided into experimental group (oral administration of chitosan phosphatidylcholine), black control group (without taking medication) and healthy control group with the age and education-matched. Serum IL-1β and TNF-α concentration among three groups were detected by using radioimmunoassay. RESULTS AND CONCLUSION: Compared with the black control group, serum IL-1β and TNF-α levels were significantly decreased in the experimental group post-treatment, and serum IL-1β and TNF-α levels in the experimental group post-treatment were significantly lower than that in the experimental group pre-treatment (P < 0.05). MMSE score was significantly improved in the experimental group post-treatment compared with experimental group pre-treatment (P < 0.05). It is indicated that chitosan phosphatidylcholine can improve cognitive function of MCI patients, lower serum IL-1β and TNFα levels, reduce inflammatory reaction and delay the MCI progression.