透明质酸改性的聚乳酸支架

背景：聚乳酸材料不具备细胞外基质材料的良好细胞亲和性能,采用化学方法将透明质酸交联制得的水凝胶具有良好的生物相容性。目的：以透明质酸对新型多孔隙率聚乳酸支架的进行改性,观察改性后支架的细胞相容性的改变。方法：采用盐析法制备出高孔隙率聚乳酸支架,采用低浓度NaOH进行表面轻度水解后,利用EDC和透明质酸进行支架的改性。结果与结论：透明质酸改性聚乳酸支架在扫描电镜下显示为多微孔的三维立体结构,孔壁及界面平滑,孔隙之间可见更细小微孔相连。改性聚乳酸支架水滴渗入较快,改性后多孔支架的保水能力与吸水能力得到明显的改善;透明质酸改性聚乳酸支架上细胞黏附及增殖优于未改性聚乳酸支架。透明质酸改性聚乳酸组软骨细胞生长密度及基质分泌更加旺盛。表明透明质酸改性聚乳酸多孔支架仍保持多孔的三维结构,其水亲和力、吸水能力、保水能力和细胞相容性均得到明显改善。关键词：透明质酸;聚乳酸;多孔支架;表面改性;水亲和力;吸水能力doi:10.3969/j.issn.1673-8225.2012.03.023

Modified polylactic acid scaffold with hyaluronic acid

BACKGROUND: Polylactic acid (HA) materials do not have the good cell affinity of extracellular matrix materials. Hydrogel cross-linked with HA by chemical methods has a good biocompatibility.  OBJECTIVE: To observe the changes in cell compatibility of new porous polylactic acid (PLA) scaffolds followed its modification with HA. METHODS: Highly porous PLA scaffolds were made by salting-out method. EDC and HA were used to modify the scaffolds following mild hydrolysis on the surface of scaffolds by NaOH with low concentration. RESULTS AND CONCLUSION: The modified PLA scaffolds with HA showed three-dimensional microporous structure underscanning electron microscopy, the pore walls and interfaces were smooth, and connection of smaller pores could be seen between the pores. Infiltration of water droplets in the modified PLA scaffolds was rapid. Water-holding capacity and water absorption capacity of the scaffolds were significantly improved. Cell adhesion and proliferation in modified scaffold were better than those unmodified. Density of cartilage cell growth and matrix secretion were more vigorous in the modified PLA scaffolds with HA. The porous three-dimensional structure of PLA scaffolds did not change after their modification with HA, and their water affinity, water absorption capacity, water capacity and biocompatibility were obviously improved after modification.