双醋瑞因对白细胞介素1β诱导软骨细胞凋亡的影响*

背景：双醋瑞因是一种新型的白细胞介素1阻滞剂,其是否对软骨细胞的凋亡有抑制作用以及是否通过细胞信号转导通路发挥作用的基础应用研究较少。 目的：观察双醋瑞因对体外白细胞介素1β诱导大鼠关节软骨细胞凋亡的影响。 方法：体外培养SD大鼠关节软骨细胞,苏木精-伊红染色和Ⅱ型胶原免疫荧光染色鉴定软骨细胞,用白细胞介素1β诱导大鼠关节软骨细胞凋亡模型,再用10-4,10-5,10-6 mol/L双醋瑞因干预培养。 结果与结论：10-4 mol/L和10-5 mol/L的双醋瑞因可降低白细胞介素1β诱导的软骨细胞凋亡率(P < 0.01和P < 0.05)。      10-5 mol/L双醋瑞因能够显著抑制白细胞介素1β诱导p38磷酸化(P < 0.01)。双醋瑞因干预的软骨细胞中p38 mRNA的表达量较模型组下降0.38倍(P < 0.01)。结果证实,双醋瑞因能够抑制软骨细胞中p38蛋白和基因的表达,从而抑制白细胞介素1β诱导关节软骨细胞凋亡。

Effects of Diacerein on apoptosis of chondroctyes induced by interleukin-1 beta

BACKGROUND: Diacerein is a novel interleukin-1 blockers, researches regarding whether it can inhibit the apoptosis of chondrocytes and whether it plays the role through signaling pathway or not are rately. OBJECTIVE: To investigate the effects of Diacerein on the apoptosis of chondroctyes induced by interleukin-1 beta (IL-1β). METHODS: Articular chondrocytes of Sprague Dawley rats were cultured in vitro and identified by hematoxylin-eosin staining and immunofluorescence staining of collagen typeⅡ. The rats apoptosis model of articular chondrocytes was induced by IL-1β, and then treated with different concentrations of diacerein (10-4, 10-5, 10-6 mol/L). RESULTS AND CONCLUSION: Diacerein with the concentrations of 10-4 mol/L and 10-5 mol/L could decrease the apoptotic rate of chondrocytes which induced by IL-1β (P < 0.01, P < 0.05). Diacerein with the concentrations of 10-5 mol/L could inhibit phosphorylation-p38 which induced by IL-1β (P < 0.01). Compared with the control group, the expression of p38 mRNA of model group was decreased by 3.78 times (P < 0.01). Diacerein can inhibit IL-1β-induced early apoptosis. The possible mechanism is related to the effects of Diacerein on the inhibition of the expression the protein and gene of p38.